skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants

Abstract

Because cellulosic ethanol production remains cost-prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash ethanol production costs through process integration. Clostridium thermocellum, a CBP microorganism, organizes its enzymes in a multi-enzyme complex - a stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit the extent of biomass deconstruction. Here in this study, six Populus were treated with C. thermocellum (ATCC 27405) and characterized to determine structural changes that resulted from CBP. The 2D HSQC NMR spectra of lignin-enriched residues revealed that higher S/G ratio (2.6) and fewer carbon-carbon interunit linkages (generally 2–5%) were present in the top performing poplar. Furthermore, cellulose degree of polymerization data suggests that C. thermocellum likely circumvents long chain cellulose, while cellulose crystallinity and hemicellulose molecular weight data do not provide a direct indication of features connected to recalcitrance. Hence, C. thermocellum is similarly impacted by the proposed lignin properties that negatively impact biomass deconstruction using fungal enzymes.

Authors:
 [1];  [2]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [3];  [2]; ORCiD logo [2]; ORCiD logo [2];  [2]; ORCiD logo [4]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Georgia Inst. of Technology, Atlanta, GA (United States). Dept. of Chemistry and Biochemistry
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  3. Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon at Wildlife, and Fisheries, Dept. of Chemical and Biomolecular Engineering & Department of Forestry; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  4. Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon at Wildlife, and Fisheries, Dept. of Chemical and Biomolecular Engineering & Department of Forestry; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1410909
Alternate Identifier(s):
OSTI ID: 1410886
Grant/Contract Number:
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Chemistry Select
Additional Journal Information:
Journal Volume: 2; Journal Issue: 33; Journal ID: ISSN 2365-6549
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; cellulosic ethanol; Clostridium thermocellum; lignin; Populus trichocarpa; recalcitrance

Citation Formats

Akinosho, Hannah, Yee, Kelsey, Rodriguez, Miguel, Muchero, Wellington, Yoo, Chang Geun, Li, Mi, Thompson, Olivia, Pu, Yunqiao Joseph, Brown, Steven D., Mielenz, Johnathan R., and Ragauskas, Arthur J.. Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants. United States: N. p., 2017. Web. doi:10.1002/slct.201701572.
Akinosho, Hannah, Yee, Kelsey, Rodriguez, Miguel, Muchero, Wellington, Yoo, Chang Geun, Li, Mi, Thompson, Olivia, Pu, Yunqiao Joseph, Brown, Steven D., Mielenz, Johnathan R., & Ragauskas, Arthur J.. Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants. United States. doi:10.1002/slct.201701572.
Akinosho, Hannah, Yee, Kelsey, Rodriguez, Miguel, Muchero, Wellington, Yoo, Chang Geun, Li, Mi, Thompson, Olivia, Pu, Yunqiao Joseph, Brown, Steven D., Mielenz, Johnathan R., and Ragauskas, Arthur J.. Tue . "Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants". United States. doi:10.1002/slct.201701572.
@article{osti_1410909,
title = {Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants},
author = {Akinosho, Hannah and Yee, Kelsey and Rodriguez, Miguel and Muchero, Wellington and Yoo, Chang Geun and Li, Mi and Thompson, Olivia and Pu, Yunqiao Joseph and Brown, Steven D. and Mielenz, Johnathan R. and Ragauskas, Arthur J.},
abstractNote = {Because cellulosic ethanol production remains cost-prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash ethanol production costs through process integration. Clostridium thermocellum, a CBP microorganism, organizes its enzymes in a multi-enzyme complex - a stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit the extent of biomass deconstruction. Here in this study, six Populus were treated with C. thermocellum (ATCC 27405) and characterized to determine structural changes that resulted from CBP. The 2D HSQC NMR spectra of lignin-enriched residues revealed that higher S/G ratio (2.6) and fewer carbon-carbon interunit linkages (generally 2–5%) were present in the top performing poplar. Furthermore, cellulose degree of polymerization data suggests that C. thermocellum likely circumvents long chain cellulose, while cellulose crystallinity and hemicellulose molecular weight data do not provide a direct indication of features connected to recalcitrance. Hence, C. thermocellum is similarly impacted by the proposed lignin properties that negatively impact biomass deconstruction using fungal enzymes.},
doi = {10.1002/slct.201701572},
journal = {Chemistry Select},
number = 33,
volume = 2,
place = {United States},
year = {Tue Nov 21 00:00:00 EST 2017},
month = {Tue Nov 21 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on November 21, 2018
Publisher's Version of Record

Save / Share: