Mix-and-diffuse serial synchrotron crystallography

Beyerlein, Kenneth R.; Dierksmeyer, Dennis; Mariani, Valerio; Kuhn, Manuela; Sarrou, Iosifina; Ottaviano, Angelica; Awel, Salah; Knoska, Juraj; Fuglerud, Silje; Jönsson, Olof; Stern, Stephan; Wiedorn, Max O.; Yefanov, Oleksandr; Adriano, Luigi; Bean, Richard; Burkhardt, Anja; Fischer, Pontus; Heymann, Michael; Horke, Daniel A.; Jungnickel, Katharina E. J.; Kovaleva, Elena; Lorbeer, Olga; Metz, Markus; Meyer, Jan; Morgan, Andrew; Pande, Kanupriya; Panneerselvam, Saravanan; Seuring, Carolin; Tolstikova, Aleksandra; Lieske, Julia; Aplin, Steve; Roessle, Manfred; White, Thomas A.; Chapman, Henry N.; Meents, Alke; Oberthuer, Dominik

doi:10.1107/S2052252517013124

Title: Mix-and-diffuse serial synchrotron crystallography

Journal Article · Mon Oct 09 00:00:00 EDT 2017 · IUCrJ

DOI:https://doi.org/10.1107/S2052252517013124· OSTI ID:1410554

^[1]; Dierksmeyer, Dennis ^[1];

^[1]; Kuhn, Manuela ^[1]; Sarrou, Iosifina ^[1];

^[2]; Awel, Salah ^[3];

^[3];

^[4];

^[5]; Stern, Stephan ^[6];

^[3];

^[1]; Adriano, Luigi ^[1];

^[1]; Burkhardt, Anja ^[1]; Fischer, Pontus ^[1];

^[1];

^[3];

^[7] more »

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
California State Univ., Northridge, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Norwegian Univ. of Science and Technology, Trondheim (Norway)
Uppsala Univ., Uppsala (Sweden)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European X-ray Free-Electron Laser Facility GmbH (XFEL), Schenefeld (Germany)
Univ. of Oxford, Oxford (England)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Fachhochschule Lubeck, Lubeck (Germany)

Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. Here, the success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

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Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515; FP7/2007-2013; ERC-614507-Küpper; DFG-EXC1074

OSTI ID:: 1410554

Journal Information:: IUCrJ, Vol. 4, Issue 6; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 75 works

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On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02620	text	January 2019
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek International Union of Crystallography (IUCr) https://doi.org/10.1107/s205225251900263x/ti5014sup1.pdf	text	January 2019
Serial protein crystallography in an electron microscope Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram Nature Publishing Group UK https://doi.org/10.15480/882.2722	text	January 2020
Ultracompact 3D microfluidics for time-resolved structural biology Knoska, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00623	text	January 2020
Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography Olmos, Jose L.; Pandey, Suraj; Martin-Garcia, Jose M. BMC Biology, Vol. 16, Issue 1 https://doi.org/10.1186/s12915-018-0524-5	journal	May 2018
Megahertz serial crystallography Wiedorn, Max Oliver; Oberthür, Dominik; Bean, Richard Nature Publishing Group UK https://doi.org/10.15480/882.2175	text	January 2018
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography Botha, S.; Baitan, D.; Jungnickel, K. E. J. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-05499	text	January 2018

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