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Title: Chemical basis for the detoxification of cisplatin-derived hydrolysis products by sodium thiosulfate

Authors:
; ; ; ; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1410351
Grant/Contract Number:
AC02-76SF00515
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Journal of Inorganic Biochemistry
Additional Journal Information:
Journal Volume: 162; Journal Issue: C; Related Information: CHORUS Timestamp: 2017-11-25 19:21:56; Journal ID: ISSN 0162-0134
Publisher:
Elsevier
Country of Publication:
United States
Language:
English

Citation Formats

Sooriyaarachchi, Melani, Gailer, Jürgen, Dolgova, Natalia V., Pickering, Ingrid J., and George, Graham N. Chemical basis for the detoxification of cisplatin-derived hydrolysis products by sodium thiosulfate. United States: N. p., 2016. Web. doi:10.1016/j.jinorgbio.2016.06.012.
Sooriyaarachchi, Melani, Gailer, Jürgen, Dolgova, Natalia V., Pickering, Ingrid J., & George, Graham N. Chemical basis for the detoxification of cisplatin-derived hydrolysis products by sodium thiosulfate. United States. doi:10.1016/j.jinorgbio.2016.06.012.
Sooriyaarachchi, Melani, Gailer, Jürgen, Dolgova, Natalia V., Pickering, Ingrid J., and George, Graham N. Thu . "Chemical basis for the detoxification of cisplatin-derived hydrolysis products by sodium thiosulfate". United States. doi:10.1016/j.jinorgbio.2016.06.012.
@article{osti_1410351,
title = {Chemical basis for the detoxification of cisplatin-derived hydrolysis products by sodium thiosulfate},
author = {Sooriyaarachchi, Melani and Gailer, Jürgen and Dolgova, Natalia V. and Pickering, Ingrid J. and George, Graham N.},
abstractNote = {},
doi = {10.1016/j.jinorgbio.2016.06.012},
journal = {Journal of Inorganic Biochemistry},
number = C,
volume = 162,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 2016},
month = {Thu Sep 01 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.jinorgbio.2016.06.012

Citation Metrics:
Cited by: 2works
Citation information provided by
Web of Science

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  • Crystals of HEWL with cisplatin and HEWL with carboplatin grown in sodium iodide conditions both show a partial chemical transformation of cisplatin or carboplatin to a transiodoplatin (PtI{sub 2}X{sub 2}) form. The binding is only at the N{sup δ} atom of His15. A further Pt species (PtI{sub 3}X) is also seen, in both cases bound in a crevice between symmetry-related protein molecules. Cisplatin and carboplatin are platinum anticancer agents that are used to treat a variety of cancers. Previous X-ray crystallographic studies of carboplatin binding to histidine in hen egg-white lysozyme (HEWL) showed a partial chemical conversion of carboplatin tomore » cisplatin owing to the high sodium chloride concentration used in the crystallization conditions. Also, the co-crystallization of HEWL with carboplatin in sodium bromide conditions resulted in the partial conversion of carboplatin to the transbromoplatin form, with a portion of the cyclobutanedicarboxylate (CBDC) moiety still present. The results of the co-crystallization of HEWL with cisplatin or carboplatin in sodium iodide conditions are now reported in order to determine whether the cisplatin and carboplatin converted to the iodo form, and whether this took place in a similar way to the partial conversion of carboplatin to cisplatin in NaCl conditions or to transbromoplatin in NaBr conditions as seen previously. It is reported here that a partial chemical transformation has taken place to a transplatin form for both ligands. The NaI-grown crystals belonged to the monoclinic space group P2{sub 1} with two molecules in the asymmetric unit. The chemically transformed cisplatin and carboplatin bind to both His15 residues, i.e. in each asymmetric unit. The binding is only at the N{sup δ} atom of His15. A third platinum species is also seen in both conditions bound in a crevice between symmetry-related molecules. Here, the platinum is bound to three I atoms identified based on their anomalous difference electron densities and their refined occupancies, with the fourth bound atom being a Cl atom (in the cisplatin case) or a portion of the CBDC moiety (in the carboplatin case)« less