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U.S. Department of Energy
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Multiplane optical microscope

Patent ·
OSTI ID:1410279

This disclosure provides systems, methods, and apparatus related to optical microscopy. In one aspect, an apparatus includes a sample holder, a first objective lens, a plurality of optical components, a second objective lens, and a mirror. The apparatus may directly image a cross-section of a sample oblique to or parallel to the optical axis of the first objective lens, without scanning.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
9,823,457
Application Number:
14/591,760
OSTI ID:
1410279
Country of Publication:
United States
Language:
English

References (16)

Aberration-free optical refocusing in high numerical aperture microscopy journal January 2007
An optical technique for remote focusing in microscopy journal February 2008
Optically sectioned imaging by oblique plane microscopy journal January 2008
Agitation-free multiphoton microscopy of oblique planes journal January 2011
New Angles on Neuronal Dendrites In Vivo journal December 2007
Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells journal April 2009
Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning journal November 2011
Imaging in high-aperture optical systems journal January 1987
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy journal August 2004
High-speed 2D and 3D fluorescence microscopy of cardiac myocytes journal January 2011
Resolution of oblique-plane images in sectioning microscopy journal January 2011
Real-time slit scanning microscopy in the meridional plane journal January 2009
Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates journal February 2012
Axial Plane Optical Microscopy journal December 2014
Vectorial point spread function and optical transfer function in oblique plane imaging journal January 2014
Inclined selective plane illumination microscopy adaptor for conventional microscopes journal June 2012

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