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Title: Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Abstract

Here, to develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Clostridium thermocellum parent Δ hpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δ hpt strain. A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

Authors:
 [1]; ORCiD logo [2]; ORCiD logo [2];  [3];  [3]; ORCiD logo [2]; ORCiD logo [4]
  1. Morgan State Univ., Baltimore, MD (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Lab., Oak Ridge, TN (United States). BioEnergy Science Center
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Lab., Oak Ridge, TN (United States). BioEnergy Science Center; LanzaTech, Inc., Skokie, IL (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1409256
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Biotechnology Letters
Additional Journal Information:
Journal Volume: 40; Journal Issue: 2; Journal ID: ISSN 0141-5492
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; 60 APPLIED LIFE SCIENCES; Bioethanol; Consolidated bioprocessing; High throughput screening; Lignocellulosic biomass; Microplate assay

Citation Formats

Oguntimein, Gbekeloluwa B., Rodriguez, Jr., Miguel, Dumitrache, Alexandru, Shollenberger, Todd, Decker, Stephen R., Davison, Brian H., and Brown, Steven D. Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum. United States: N. p., 2017. Web. doi:10.1007/s10529-017-2467-2.
Oguntimein, Gbekeloluwa B., Rodriguez, Jr., Miguel, Dumitrache, Alexandru, Shollenberger, Todd, Decker, Stephen R., Davison, Brian H., & Brown, Steven D. Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum. United States. doi:10.1007/s10529-017-2467-2.
Oguntimein, Gbekeloluwa B., Rodriguez, Jr., Miguel, Dumitrache, Alexandru, Shollenberger, Todd, Decker, Stephen R., Davison, Brian H., and Brown, Steven D. Thu . "Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum". United States. doi:10.1007/s10529-017-2467-2. https://www.osti.gov/servlets/purl/1409256.
@article{osti_1409256,
title = {Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum},
author = {Oguntimein, Gbekeloluwa B. and Rodriguez, Jr., Miguel and Dumitrache, Alexandru and Shollenberger, Todd and Decker, Stephen R. and Davison, Brian H. and Brown, Steven D.},
abstractNote = {Here, to develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.},
doi = {10.1007/s10529-017-2467-2},
journal = {Biotechnology Letters},
issn = {0141-5492},
number = 2,
volume = 40,
place = {United States},
year = {2017},
month = {11}
}

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