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Title: Method for delivery of small molecules and proteins across the cell wall of algae using molecular transporters

Abstract

The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1409013
Patent Number(s):
9,816,066
Application Number:
14/396,274
Assignee:
The Regents of the University of California (Oakland, CA); The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Mar 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Geihe, Erika, Trantow, Brian, Wender, Paul, Hyman, Joel M., and Parvin, Bahram. Method for delivery of small molecules and proteins across the cell wall of algae using molecular transporters. United States: N. p., 2017. Web.
Geihe, Erika, Trantow, Brian, Wender, Paul, Hyman, Joel M., & Parvin, Bahram. Method for delivery of small molecules and proteins across the cell wall of algae using molecular transporters. United States.
Geihe, Erika, Trantow, Brian, Wender, Paul, Hyman, Joel M., and Parvin, Bahram. 2017. "Method for delivery of small molecules and proteins across the cell wall of algae using molecular transporters". United States. https://www.osti.gov/servlets/purl/1409013.
@article{osti_1409013,
title = {Method for delivery of small molecules and proteins across the cell wall of algae using molecular transporters},
author = {Geihe, Erika and Trantow, Brian and Wender, Paul and Hyman, Joel M. and Parvin, Bahram},
abstractNote = {The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.},
doi = {},
url = {https://www.osti.gov/biblio/1409013}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {11}
}

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