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Title: Systems Biology of Lipid Body Formation in the Green Alga Chlamydomonas reinhardtii

Abstract

The project aimed to deepen our understanding of alga triacylglycerol (TAG) production to undergird explorations of using algal TAG as a source of biodiesel fuel. Our published contributions included the following: 1) Development of a rapid assay for TAG in algal cultures which was widely distributed to the algal community. 2) A comprehensive transcriptome analysis of the development of the ultra-high-TAG “obese” phenotype In Chlamydomonas reinhardtii. 3) A comprehensive biochemical and ultrastructural analysis of the cell wall of Nannochloropsis gaditana, whose walls render it both growth-hardy and difficult to rupture for TAG recovery. A manuscript in preparation considers the autophagy response in C. reinhardtii and its entrance into stationary phase, both having an impact on TAG production.

Authors:
 [1]
  1. Washington Univ., St. Louis, MO (United States)
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1408918
Report Number(s):
DOE-WashU-06837
DOE Contract Number:  
SC0006873
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; algae; biodiesel; triacylglyceride; sustainable energy

Citation Formats

Goodenough, Ursula. Systems Biology of Lipid Body Formation in the Green Alga Chlamydomonas reinhardtii. United States: N. p., 2017. Web. doi:10.2172/1408918.
Goodenough, Ursula. Systems Biology of Lipid Body Formation in the Green Alga Chlamydomonas reinhardtii. United States. doi:10.2172/1408918.
Goodenough, Ursula. Fri . "Systems Biology of Lipid Body Formation in the Green Alga Chlamydomonas reinhardtii". United States. doi:10.2172/1408918. https://www.osti.gov/servlets/purl/1408918.
@article{osti_1408918,
title = {Systems Biology of Lipid Body Formation in the Green Alga Chlamydomonas reinhardtii},
author = {Goodenough, Ursula},
abstractNote = {The project aimed to deepen our understanding of alga triacylglycerol (TAG) production to undergird explorations of using algal TAG as a source of biodiesel fuel. Our published contributions included the following: 1) Development of a rapid assay for TAG in algal cultures which was widely distributed to the algal community. 2) A comprehensive transcriptome analysis of the development of the ultra-high-TAG “obese” phenotype In Chlamydomonas reinhardtii. 3) A comprehensive biochemical and ultrastructural analysis of the cell wall of Nannochloropsis gaditana, whose walls render it both growth-hardy and difficult to rupture for TAG recovery. A manuscript in preparation considers the autophagy response in C. reinhardtii and its entrance into stationary phase, both having an impact on TAG production.},
doi = {10.2172/1408918},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {11}
}