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Title: Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells

Abstract

Exposures to various DNA damaging agents can deregulate a wide array of critical mechanisms that maintain genome integrity. It is unclear how these processes are impacted by one's age at the time of exposure and the complexity of the DNA lesion. To clarify this, we employed radiation as a tool to generate simple and complex lesions in normal primary human mammary epithelial cells derived from women of various ages. We hypothesized that genomic instability in the progeny of older cells exposed to complex damages will be exacerbated by age-associated deterioration in function and accentuate age-related cancer predisposition. Centrosome aberrations and changes in stem cell numbers were examined to assess cancer susceptibility. Our data show that the frequency of centrosome aberrations proportionately increases with age following complex damage causing exposures. However, a dose-dependent increase in stem cell numbers was independent of both age and the nature of the insult. Phospho-protein signatures provide mechanistic clues to signaling networks implicated in these effects. Together these studies suggest that complex damage can threaten the genome stability of the stem cell population in older people. Propagation of this instability is subject to influence by the microenvironment and will ultimately define cancer risk in the oldermore » population.« less

Authors:
 [1];  [1];  [1];  [2];  [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. City of Hope National Medical Center, Duarte, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1408407
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Aging
Additional Journal Information:
Journal Volume: 9; Journal Issue: 3; Journal ID: ISSN 1945-4589
Publisher:
Impact Journals
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Sridharan, Deepa M., Enerio, Shiena, Stampfer, Martha M., LaBarge, Mark A., and Pluth, Janice M. Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells. United States: N. p., 2017. Web. doi:10.18632/aging.101183.
Sridharan, Deepa M., Enerio, Shiena, Stampfer, Martha M., LaBarge, Mark A., & Pluth, Janice M. Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells. United States. doi:10.18632/aging.101183.
Sridharan, Deepa M., Enerio, Shiena, Stampfer, Martha M., LaBarge, Mark A., and Pluth, Janice M. Tue . "Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells". United States. doi:10.18632/aging.101183. https://www.osti.gov/servlets/purl/1408407.
@article{osti_1408407,
title = {Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells},
author = {Sridharan, Deepa M. and Enerio, Shiena and Stampfer, Martha M. and LaBarge, Mark A. and Pluth, Janice M.},
abstractNote = {Exposures to various DNA damaging agents can deregulate a wide array of critical mechanisms that maintain genome integrity. It is unclear how these processes are impacted by one's age at the time of exposure and the complexity of the DNA lesion. To clarify this, we employed radiation as a tool to generate simple and complex lesions in normal primary human mammary epithelial cells derived from women of various ages. We hypothesized that genomic instability in the progeny of older cells exposed to complex damages will be exacerbated by age-associated deterioration in function and accentuate age-related cancer predisposition. Centrosome aberrations and changes in stem cell numbers were examined to assess cancer susceptibility. Our data show that the frequency of centrosome aberrations proportionately increases with age following complex damage causing exposures. However, a dose-dependent increase in stem cell numbers was independent of both age and the nature of the insult. Phospho-protein signatures provide mechanistic clues to signaling networks implicated in these effects. Together these studies suggest that complex damage can threaten the genome stability of the stem cell population in older people. Propagation of this instability is subject to influence by the microenvironment and will ultimately define cancer risk in the older population.},
doi = {10.18632/aging.101183},
journal = {Aging},
number = 3,
volume = 9,
place = {United States},
year = {Tue Feb 28 00:00:00 EST 2017},
month = {Tue Feb 28 00:00:00 EST 2017}
}

Journal Article:
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