Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Oguntimein, Gbekeloluwa B.; Rodriguez, Jr., Miguel; Dumitrache, Alexandru; Shollenberger, Todd; Decker, Stephen R.; Davison, Brian H.; Brown, Steven D.

doi:10.1007/s10529-017-2467-2

Title: Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Journal Article · Thu Nov 09 00:00:00 EST 2017 · Biotechnology Letters

DOI:https://doi.org/10.1007/s10529-017-2467-2· OSTI ID:1408146

Oguntimein, Gbekeloluwa B.; Rodriguez, Jr., Miguel; Dumitrache, Alexandru; Shollenberger, Todd; Decker, Stephen R.; Davison, Brian H.; Brown, Steven D.

Here, to develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain. A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308; AC05-00OR22725

OSTI ID:: 1408146

Alternate ID(s):: OSTI ID: 1409256; OSTI ID: 1409731

Report Number(s):: NREL/JA-2700-70541; PII: 2467

Journal Information:: Biotechnology Letters, Journal Name: Biotechnology Letters Vol. 40 Journal Issue: 2; ISSN 0141-5492

Publisher:: SpringerCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 2 works

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References (15)

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59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
60 APPLIED LIFE SCIENCES
Bioethanol
Consolidated bioprocessing
High throughput screening
Lignocellulosic biomass
Microplate assay
bioethanol
consolidated bioprocessing
high throughput screening
lignocellulosic biomass
microplate assay

Title: Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

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