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Title: Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain

Abstract

Two nonstructural proteins encoded byZika virusstrain MR766 RNA, a methyltransferase and a helicase, were crystallized and their structures were solved and refined at 2.10 and 2.01 Å resolution, respectively. The NS5 methyltransferase contains a boundS-adenosyl-L-methionine (SAM) co-substrate. The NS3 helicase is in the apo form. Comparison with published crystal structures of the helicase in the apo, nucleotide-bound and single-stranded RNA (ssRNA)-bound states suggests that binding of ssRNA to the helicase may occur through conformational selection rather than induced fit.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
DOE - BASIC ENERGY SCIENCES
OSTI Identifier:
1408119
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section D. Structural Biology
Additional Journal Information:
Journal Volume: 73; Journal Issue: 9; Journal ID: ISSN 2059-7983
Publisher:
IUCr
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bukrejewska, Malgorzata, Derewenda, Urszula, Radwanska, Malwina, Engel, Daniel A., and Derewenda, Zygmunt S. Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain. United States: N. p., 2017. Web. doi:10.1107/S2059798317010737.
Bukrejewska, Malgorzata, Derewenda, Urszula, Radwanska, Malwina, Engel, Daniel A., & Derewenda, Zygmunt S. Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain. United States. doi:10.1107/S2059798317010737.
Bukrejewska, Malgorzata, Derewenda, Urszula, Radwanska, Malwina, Engel, Daniel A., and Derewenda, Zygmunt S. Tue . "Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain". United States. doi:10.1107/S2059798317010737.
@article{osti_1408119,
title = {Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain},
author = {Bukrejewska, Malgorzata and Derewenda, Urszula and Radwanska, Malwina and Engel, Daniel A. and Derewenda, Zygmunt S.},
abstractNote = {Two nonstructural proteins encoded byZika virusstrain MR766 RNA, a methyltransferase and a helicase, were crystallized and their structures were solved and refined at 2.10 and 2.01 Å resolution, respectively. The NS5 methyltransferase contains a boundS-adenosyl-L-methionine (SAM) co-substrate. The NS3 helicase is in the apo form. Comparison with published crystal structures of the helicase in the apo, nucleotide-bound and single-stranded RNA (ssRNA)-bound states suggests that binding of ssRNA to the helicase may occur through conformational selection rather than induced fit.},
doi = {10.1107/S2059798317010737},
journal = {Acta Crystallographica. Section D. Structural Biology},
issn = {2059-7983},
number = 9,
volume = 73,
place = {United States},
year = {2017},
month = {8}
}