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Title: Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks

Abstract

In order to enable the large-scale production of biofuels or chemicals from lignocellulosic biomass, a consistent and affordable year-round supply of lignocellulosic feedstocks is essential. Feedstock blending and/or densification offers one promising solution to overcome current challenges on biomass supply, i.e., low energy and bulk densities and significant compositional variations. Therefore, it is imperative to develop conversion technologies that can process mixed pelleted biomass feedstocks with minimal negative impact in terms of overall performance of the relevant biorefinery unit operations: pretreatment, fermentable sugar production, and fuel titers. We processed the mixture of four feedstocks—corn stover, switchgrass, lodgepole pine, and eucalyptus (1:1:1:1 on dry weight basis)—in flour and pellet form using ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate, dilute sulfuric acid (DA), and soaking in aqueous ammonia (SAA) pretreatments. Commercial enzyme mixtures, including cellulases and hemicellulases, were then applied to these pretreated feedstocks at low to moderate enzyme loadings to determine hydrolysis efficiency. Results show significant variations on the chemical composition, crystallinity, and enzymatic digestibility of the pretreated feedstocks across the different pretreatment technologies studied. The advanced biofuel isopentenol was produced during simultaneous saccharification and fermentation (SSF) of pretreated feedstocks using an engineered Escherichia coli strain. Results show that IL pretreatment liberates themore » most sugar during enzymatic saccharification, and in turn led to the highest isopentenol titer as compared to DA and SAA pretreatments. This study provides insights on developing biorefinery technologies that produce advanced biofuels based on mixed feedstock streams.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1407389
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 8; Journal Issue: 3; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Shi, Jian, George, Kevin W., Sun, Ning, He, Wei, Li, Chenlin, Stavila, Vitalie, Keasling, Jay D., Simmons, Blake A., Lee, Taek Soon, and Singh, Seema. Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks. United States: N. p., 2015. Web. doi:10.1007/s12155-015-9588-z.
Shi, Jian, George, Kevin W., Sun, Ning, He, Wei, Li, Chenlin, Stavila, Vitalie, Keasling, Jay D., Simmons, Blake A., Lee, Taek Soon, & Singh, Seema. Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks. United States. doi:10.1007/s12155-015-9588-z.
Shi, Jian, George, Kevin W., Sun, Ning, He, Wei, Li, Chenlin, Stavila, Vitalie, Keasling, Jay D., Simmons, Blake A., Lee, Taek Soon, and Singh, Seema. Sat . "Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks". United States. doi:10.1007/s12155-015-9588-z.
@article{osti_1407389,
title = {Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks},
author = {Shi, Jian and George, Kevin W. and Sun, Ning and He, Wei and Li, Chenlin and Stavila, Vitalie and Keasling, Jay D. and Simmons, Blake A. and Lee, Taek Soon and Singh, Seema},
abstractNote = {In order to enable the large-scale production of biofuels or chemicals from lignocellulosic biomass, a consistent and affordable year-round supply of lignocellulosic feedstocks is essential. Feedstock blending and/or densification offers one promising solution to overcome current challenges on biomass supply, i.e., low energy and bulk densities and significant compositional variations. Therefore, it is imperative to develop conversion technologies that can process mixed pelleted biomass feedstocks with minimal negative impact in terms of overall performance of the relevant biorefinery unit operations: pretreatment, fermentable sugar production, and fuel titers. We processed the mixture of four feedstocks—corn stover, switchgrass, lodgepole pine, and eucalyptus (1:1:1:1 on dry weight basis)—in flour and pellet form using ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate, dilute sulfuric acid (DA), and soaking in aqueous ammonia (SAA) pretreatments. Commercial enzyme mixtures, including cellulases and hemicellulases, were then applied to these pretreated feedstocks at low to moderate enzyme loadings to determine hydrolysis efficiency. Results show significant variations on the chemical composition, crystallinity, and enzymatic digestibility of the pretreated feedstocks across the different pretreatment technologies studied. The advanced biofuel isopentenol was produced during simultaneous saccharification and fermentation (SSF) of pretreated feedstocks using an engineered Escherichia coli strain. Results show that IL pretreatment liberates the most sugar during enzymatic saccharification, and in turn led to the highest isopentenol titer as compared to DA and SAA pretreatments. This study provides insights on developing biorefinery technologies that produce advanced biofuels based on mixed feedstock streams.},
doi = {10.1007/s12155-015-9588-z},
journal = {BioEnergy Research},
issn = {1939-1234},
number = 3,
volume = 8,
place = {United States},
year = {2015},
month = {2}
}

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