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Title: Molecular Sensing Using Hyperpolarized Xenon NMR Spectroscopy

Abstract

Molecular imaging is defined as the determination of the spatial location and concentration of specific molecules in a sample of interest. Sophisticated modern magnetic resonance imaging machines can collect NMR spectra from small-volume elements within a sample, enabling local chemical analysis. However, abundant water and fat signals limit detection of metabolites to near mm concentrations. Alternatively, targeted relaxation contrast agents enhance the relaxation of the strong water signal where they bind. A comparison of images with and without a contrast agent shows the target distribution, but high mm concentrations are needed. We have developed an approach that exploits the strong signals of hyperpolarized 129 Xe (an inert reporter introduced for imaging). The imaging contrast agents are composed of a biological recognition motif to localize the agent (antibodies or aptamers) and covalently tethered cryptophane cages. Xenon binds to the cryptophane and though chemical exchange saturation transfer creates contrast in a xenon image. Imaging agents can deliver many cages per target, giving detection limits in the pm concentration range. The evolution and principles of this approach are described.

Authors:
 [1];  [1];  [1];  [2]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division
  2. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1407213
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Israel Journal of Chemistry
Additional Journal Information:
Journal Volume: 54; Journal Issue: 1-2; Journal ID: ISSN 0021-2148
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Palaniappan, Krishnan K., Francis, Matthew B., Pines, Alexander, and Wemmer, David E. Molecular Sensing Using Hyperpolarized Xenon NMR Spectroscopy. United States: N. p., 2014. Web. doi:10.1002/ijch.201300128.
Palaniappan, Krishnan K., Francis, Matthew B., Pines, Alexander, & Wemmer, David E. Molecular Sensing Using Hyperpolarized Xenon NMR Spectroscopy. United States. doi:10.1002/ijch.201300128.
Palaniappan, Krishnan K., Francis, Matthew B., Pines, Alexander, and Wemmer, David E. Sat . "Molecular Sensing Using Hyperpolarized Xenon NMR Spectroscopy". United States. doi:10.1002/ijch.201300128.
@article{osti_1407213,
title = {Molecular Sensing Using Hyperpolarized Xenon NMR Spectroscopy},
author = {Palaniappan, Krishnan K. and Francis, Matthew B. and Pines, Alexander and Wemmer, David E.},
abstractNote = {Molecular imaging is defined as the determination of the spatial location and concentration of specific molecules in a sample of interest. Sophisticated modern magnetic resonance imaging machines can collect NMR spectra from small-volume elements within a sample, enabling local chemical analysis. However, abundant water and fat signals limit detection of metabolites to near mm concentrations. Alternatively, targeted relaxation contrast agents enhance the relaxation of the strong water signal where they bind. A comparison of images with and without a contrast agent shows the target distribution, but high mm concentrations are needed. We have developed an approach that exploits the strong signals of hyperpolarized 129 Xe (an inert reporter introduced for imaging). The imaging contrast agents are composed of a biological recognition motif to localize the agent (antibodies or aptamers) and covalently tethered cryptophane cages. Xenon binds to the cryptophane and though chemical exchange saturation transfer creates contrast in a xenon image. Imaging agents can deliver many cages per target, giving detection limits in the pm concentration range. The evolution and principles of this approach are described.},
doi = {10.1002/ijch.201300128},
journal = {Israel Journal of Chemistry},
issn = {0021-2148},
number = 1-2,
volume = 54,
place = {United States},
year = {2014},
month = {2}
}

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