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Title: Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat

Abstract

Benthic microbial ecosystems are widespread yet knowledge gaps still remain on the relationships between the diversity of species across kingdoms and productivity. Here, we ask two fundamental questions: 1) How does species diversity relate to the rates of primary and heterotrophic productivity? 2) How do diel variations in light-energy inputs influence productivity and microbiome diversity? To answer these questions, microbial mats from a magnesium sulfate hypersaline Lake were used to establish microcosms. Both the number and relatedness between bacterial and eukaryotic taxa in the microbiome were assayed via amplicon based sequencing of 16S and 18S rRNA genes over two diel cycles. These results correlated with biomass productivity obtained from substrate-specific 13C stable isotope incorporation that enabled comparisons between primary and heterotrophic productivity. Both bacterial and eukaryotic species richness and evenness were related only to the rates of 13C labeled glucose and acetate biomass incorporation. Interestingly, measures of these heterotrophic relationships changed from positive and negative correlations depending on carbon derived from glucose and acetate, respectively. Bacterial and eukaryotic diversity of this ecosystem is also controlled, in part, energy constraints imposed by changing irradiance over a diel cycle.

Authors:
ORCiD logo; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1406667
Report Number(s):
PNNL-SA-127139
Journal ID: ISSN 1574-6941; KP1601010
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: FEMS Microbiology Ecology (Online); Journal Volume: 93; Journal Issue: 10
Country of Publication:
United States
Language:
English

Citation Formats

Bernstein, Hans C., Brislawn, Colin J., Dana, Karl, Flores-Wentz, Tobias, Cory, Alexandra B., Fansler, Sarah J., Fredrickson, James K., and Moran, James J. Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat. United States: N. p., 2017. Web. doi:10.1093/femsec/fix121.
Bernstein, Hans C., Brislawn, Colin J., Dana, Karl, Flores-Wentz, Tobias, Cory, Alexandra B., Fansler, Sarah J., Fredrickson, James K., & Moran, James J. Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat. United States. doi:10.1093/femsec/fix121.
Bernstein, Hans C., Brislawn, Colin J., Dana, Karl, Flores-Wentz, Tobias, Cory, Alexandra B., Fansler, Sarah J., Fredrickson, James K., and Moran, James J. Sun . "Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat". United States. doi:10.1093/femsec/fix121.
@article{osti_1406667,
title = {Primary and heterotrophic productivity relate to multikingdom diversity in a hypersaline mat},
author = {Bernstein, Hans C. and Brislawn, Colin J. and Dana, Karl and Flores-Wentz, Tobias and Cory, Alexandra B. and Fansler, Sarah J. and Fredrickson, James K. and Moran, James J.},
abstractNote = {Benthic microbial ecosystems are widespread yet knowledge gaps still remain on the relationships between the diversity of species across kingdoms and productivity. Here, we ask two fundamental questions: 1) How does species diversity relate to the rates of primary and heterotrophic productivity? 2) How do diel variations in light-energy inputs influence productivity and microbiome diversity? To answer these questions, microbial mats from a magnesium sulfate hypersaline Lake were used to establish microcosms. Both the number and relatedness between bacterial and eukaryotic taxa in the microbiome were assayed via amplicon based sequencing of 16S and 18S rRNA genes over two diel cycles. These results correlated with biomass productivity obtained from substrate-specific 13C stable isotope incorporation that enabled comparisons between primary and heterotrophic productivity. Both bacterial and eukaryotic species richness and evenness were related only to the rates of 13C labeled glucose and acetate biomass incorporation. Interestingly, measures of these heterotrophic relationships changed from positive and negative correlations depending on carbon derived from glucose and acetate, respectively. Bacterial and eukaryotic diversity of this ecosystem is also controlled, in part, energy constraints imposed by changing irradiance over a diel cycle.},
doi = {10.1093/femsec/fix121},
journal = {FEMS Microbiology Ecology (Online)},
number = 10,
volume = 93,
place = {United States},
year = {Sun Oct 01 00:00:00 EDT 2017},
month = {Sun Oct 01 00:00:00 EDT 2017}
}