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Title: Ionic liquid-tolerant cellulase enzymes

Abstract

The present invention provides ionic liquid-tolerant cellulases and method of producing and using such cellulases. The cellulases of the invention are useful in saccharification reactions using ionic liquid treated biomass.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1406574
Patent Number(s):
9,803,182
Application Number:
14/612,201
Assignee:
The Regents of the University of California LBNL
DOE Contract Number:
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Feb 02
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Gladden, John, Park, Joshua, Singer, Steven, Simmons, Blake, and Sale, Ken. Ionic liquid-tolerant cellulase enzymes. United States: N. p., 2017. Web.
Gladden, John, Park, Joshua, Singer, Steven, Simmons, Blake, & Sale, Ken. Ionic liquid-tolerant cellulase enzymes. United States.
Gladden, John, Park, Joshua, Singer, Steven, Simmons, Blake, and Sale, Ken. 2017. "Ionic liquid-tolerant cellulase enzymes". United States. doi:. https://www.osti.gov/servlets/purl/1406574.
@article{osti_1406574,
title = {Ionic liquid-tolerant cellulase enzymes},
author = {Gladden, John and Park, Joshua and Singer, Steven and Simmons, Blake and Sale, Ken},
abstractNote = {The present invention provides ionic liquid-tolerant cellulases and method of producing and using such cellulases. The cellulases of the invention are useful in saccharification reactions using ionic liquid treated biomass.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2017,
month =
}

Patent:

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  • The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.
  • The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.
  • Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.
  • The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.