Hyperspectral imaging flow cytometer

Sinclair, Michael B.; Jones, Howland D. T.

Hyperspectral imaging flow cytometer

Patent · 25 October 2017

OSTI ID:1404931

Sinclair, Michael B.; Jones, Howland D. T.

A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: National Technology & Engineering Solutions of Sandia, LLC

Patent Number(s):: 9,797,836

Application Number:: 13/769,724

OSTI ID:: 1404931

Country of Publication:: United States

Language:: English

References (12)

Hyperspectral imaging of biological targets: The difference a high resolution spectral dimension and multivariate analysis can make Timlin, J.A.; Sinclair, M.B.; Haaland, D.M. 2004 2nd IEEE International Symposium on Biomedical Imaging: Nano to Macro (IEEE Cat No. 04EX821), Vol. 2, p. 1529-1532 https://doi.org/10.1109/ISBI.2004.1398842	conference	March 2005
Weighting hyperspectral image data for improved multivariate curve resolution results Jones, Howland D. T.; Haaland, David M.; Sinclair, Michael B. Journal of Chemometrics, Vol. 22, Issue 9, p. 482-490 https://doi.org/10.1002/cem.1170	journal	September 2008
Hyperspectral microarray scanning: impact on the accuracy and reliability of gene expression data Timlin, Jerilyn A.; Haaland, David M.; Sinclair, Michael B. BMC Genomics, Vol. 6, Article No. 72 https://doi.org/10.1186/1471-2164-6-72	journal	January 2005
Imaging multiple endogenous and exogenous fluorescent species in cells and tissues Timlin, Jerilyn A.; Nieman, Linda T.; Jones, Howland D. T. SPIE BiOS, Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, Vol. 6088, Issue 608805 https://doi.org/10.1117/12.647228	conference	February 2006
Identification and removal of contaminating fluorescence from commercial and in-house printed DNA microarrays Martinez, M. J.; Aragon, A. D.; Rodriguez, A. L. Nucleic Acids Research, Vol. 31, Issue 4, p. e18 https://doi.org/10.1093/nar/gng018	journal	February 2003
Accurate Detection of Low Levels of Fluorescence Emission in Autofluorescent Background: Francisella-Infected Macrophage Cells Davis, Ryan W.; Timlin, Jerilyn A.; Kaiser, Julia N. Microscopy and Microanalysis, Vol. 16, Issue 4, p. 478-487 https://doi.org/10.1017/S1431927610000322	journal	June 2010
Antimicrobial peptide interactions with silica bead supported bilayers and E. coli: buforin II, magainin II, and arenicin Davis, Ryan W.; Arango, Dulce C.; Jones, Howland D. T. Journal of Peptide Science, Vol. 15, Issue 8, p. 511-522 https://doi.org/10.1002/psc.1152	journal	August 2009
In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells Vermaas, W. F. J.; Timlin, J. A.; Jones, H. D. T. Proceedings of the National Academy of Sciences, Vol. 105, Issue 10, p. 4050-4055 https://doi.org/10.1073/pnas.0708090105	journal	March 2008
Design, construction, characterization, and application of a hyperspectral microarray scanner Sinclair, Michael B.; Timlin, Jerilyn A.; Haaland, David M. Applied Optics, Vol. 43, Issue 10, p. 2079-2088 https://doi.org/10.1364/AO.43.002079	journal	January 2004
Multivariate statistical analysis of time-of-flight secondary ion mass spectrometry images using AXSIA Ohlhausen, J. A.Tony; Keenan, M. R.; Kotula, P. G. Applied Surface Science, Vol. 231-232, p. 230-234 https://doi.org/10.1016/j.apsusc.2004.03.020	journal	June 2004
Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution Sutherland, Vicki L.; Timlin, Jerilyn A.; Nieman, Linda T. Journal of Neuroscience Methods, Vol. 160, Issue 1, p. 144-148 https://doi.org/10.1016/j.jneumeth.2006.08.018	journal	February 2007
Hyperspectral confocal microscope Sinclair, Michael B.; Haaland, David M.; Timlin, Jerilyn A. Applied Optics, Vol. 45, Issue 24, p. 6283-6291 https://doi.org/10.1364/AO.45.006283	journal	January 2006

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