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Title: Integrated Micro-Optical Fluorescence Detection System for Microfluidic Electrochromatography

Abstract

The authors describe the design and microfabrication of an extremely compact optical system as a key element in an integrated capillary-channel electrochromatograph with laser induced fluorescence detection. The optical design uses substrate-mode propagation within the fused silica substrate. The optical system includes a vertical cavity surface-emitting laser (VCSEL) array, two high performance microlenses and a commercial photodetector. The microlenses are multilevel diffractive optics patterned by electron beam lithography and etched by reactive ion etching in fused silica. Two generations of optical subsystems are described. The first generation design is integrated directly onto the capillary channel-containing substrate with a 6 mm separation between the VCSEL and photodetector. The second generation design separates the optical system onto its own module and the source to detector length is further compressed to 3.5 mm. The systems are designed for indirect fluorescence detection using infrared dyes. The first generation design has been tested with a 750 nm VCSEL exciting a 10{sup -4} M solution of CY-7 dye. The observed signal-to-noise ratio of better than 100:1 demonstrates that the background signal from scattered pump light is low despite the compact size of the optical system and meets the system sensitivity requirements.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Sandia National Labs., Albuquerque, NM (US); Sandia National Labs., Livermore, CA (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
14032
Report Number(s):
SAND99-2463C
TRN: AH200136%%285
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Conference
Resource Relation:
Conference: SPIE 1999 Symposium on Micromachining and Microfabrication, Santa Clara, CA (US), 09/20/1999--09/22/1999; Other Information: PBD: 16 Sep 1999
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 47 OTHER INSTRUMENTATION; DESIGN; DETECTION; FLUORESCENCE; OPTICAL SYSTEMS; SIGNAL-TO-NOISE RATIO; SILICA; SUBSTRATES; MINIATURIZATION; FABRICATION; CHROMATOGRAPHY; LASERS; MICRO-OPTICS; VCSEL; ELECTROCHROMATOGRAPHY; DIFFRACTIVE OPTICS; SUBSTRATE-MODE; MICROFLUIDICS; CHEMICAL SENSING

Citation Formats

ALLERMAN,ANDREW A., ARNOLD,DON W., ASBILL,RANDOLPH E., BAILEY,CHRISTOPHER G., CARTER,TONY RAY, KEMME,SHANALYN A., MATZKE,CAROLYN M., SAMORA,SALLY, SWEATT,WILLIAM C., WARREN,MIAL E., and WENDT,JOEL R. Integrated Micro-Optical Fluorescence Detection System for Microfluidic Electrochromatography. United States: N. p., 1999. Web.
ALLERMAN,ANDREW A., ARNOLD,DON W., ASBILL,RANDOLPH E., BAILEY,CHRISTOPHER G., CARTER,TONY RAY, KEMME,SHANALYN A., MATZKE,CAROLYN M., SAMORA,SALLY, SWEATT,WILLIAM C., WARREN,MIAL E., & WENDT,JOEL R. Integrated Micro-Optical Fluorescence Detection System for Microfluidic Electrochromatography. United States.
ALLERMAN,ANDREW A., ARNOLD,DON W., ASBILL,RANDOLPH E., BAILEY,CHRISTOPHER G., CARTER,TONY RAY, KEMME,SHANALYN A., MATZKE,CAROLYN M., SAMORA,SALLY, SWEATT,WILLIAM C., WARREN,MIAL E., and WENDT,JOEL R. Thu . "Integrated Micro-Optical Fluorescence Detection System for Microfluidic Electrochromatography". United States. https://www.osti.gov/servlets/purl/14032.
@article{osti_14032,
title = {Integrated Micro-Optical Fluorescence Detection System for Microfluidic Electrochromatography},
author = {ALLERMAN,ANDREW A. and ARNOLD,DON W. and ASBILL,RANDOLPH E. and BAILEY,CHRISTOPHER G. and CARTER,TONY RAY and KEMME,SHANALYN A. and MATZKE,CAROLYN M. and SAMORA,SALLY and SWEATT,WILLIAM C. and WARREN,MIAL E. and WENDT,JOEL R.},
abstractNote = {The authors describe the design and microfabrication of an extremely compact optical system as a key element in an integrated capillary-channel electrochromatograph with laser induced fluorescence detection. The optical design uses substrate-mode propagation within the fused silica substrate. The optical system includes a vertical cavity surface-emitting laser (VCSEL) array, two high performance microlenses and a commercial photodetector. The microlenses are multilevel diffractive optics patterned by electron beam lithography and etched by reactive ion etching in fused silica. Two generations of optical subsystems are described. The first generation design is integrated directly onto the capillary channel-containing substrate with a 6 mm separation between the VCSEL and photodetector. The second generation design separates the optical system onto its own module and the source to detector length is further compressed to 3.5 mm. The systems are designed for indirect fluorescence detection using infrared dyes. The first generation design has been tested with a 750 nm VCSEL exciting a 10{sup -4} M solution of CY-7 dye. The observed signal-to-noise ratio of better than 100:1 demonstrates that the background signal from scattered pump light is low despite the compact size of the optical system and meets the system sensitivity requirements.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {9}
}

Conference:
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