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Title: Draft Nuclear Genome, Complete Chloroplast Genome, and Complete Mitochondrial Genome for the Biofuel/Bioproduct Feedstock Species Scenedesmus obliquus Strain DOE0152z

Abstract

ABSTRACT The green alga Scenedesmus obliquus is an emerging platform species for the industrial production of biofuels. Here, we report the draft assembly and annotation for the nuclear, plastid, and mitochondrial genomes of S. obliquus strain DOE0152z.

Authors:
 [1];  [2];  [1];  [1];  [1];  [3];  [2]; ORCiD logo [3]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Brooklyn College of the City Univ. of New York (United States); City Univ. (CUNY), NY (United States)
  3. Brooklyn College of the City Univ. of New York (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1402634
Report Number(s):
LA-UR-17-24751
Journal ID: ISSN 2169-8287
Grant/Contract Number:
AC52-06NA25396
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Genome Announcements
Additional Journal Information:
Journal Volume: 5; Journal Issue: 32; Journal ID: ISSN 2169-8287
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; Biological Science; Scenedesmus; Genome

Citation Formats

Starkenburg, S. R., Polle, J. E. W., Hovde, B., Daligault, H. E., Davenport, K. W., Huang, A., Neofotis, P., and McKie-Krisberg, Z.. Draft Nuclear Genome, Complete Chloroplast Genome, and Complete Mitochondrial Genome for the Biofuel/Bioproduct Feedstock Species Scenedesmus obliquus Strain DOE0152z. United States: N. p., 2017. Web. doi:10.1128/genomeA.00617-17.
Starkenburg, S. R., Polle, J. E. W., Hovde, B., Daligault, H. E., Davenport, K. W., Huang, A., Neofotis, P., & McKie-Krisberg, Z.. Draft Nuclear Genome, Complete Chloroplast Genome, and Complete Mitochondrial Genome for the Biofuel/Bioproduct Feedstock Species Scenedesmus obliquus Strain DOE0152z. United States. doi:10.1128/genomeA.00617-17.
Starkenburg, S. R., Polle, J. E. W., Hovde, B., Daligault, H. E., Davenport, K. W., Huang, A., Neofotis, P., and McKie-Krisberg, Z.. Thu . "Draft Nuclear Genome, Complete Chloroplast Genome, and Complete Mitochondrial Genome for the Biofuel/Bioproduct Feedstock Species Scenedesmus obliquus Strain DOE0152z". United States. doi:10.1128/genomeA.00617-17.
@article{osti_1402634,
title = {Draft Nuclear Genome, Complete Chloroplast Genome, and Complete Mitochondrial Genome for the Biofuel/Bioproduct Feedstock Species Scenedesmus obliquus Strain DOE0152z},
author = {Starkenburg, S. R. and Polle, J. E. W. and Hovde, B. and Daligault, H. E. and Davenport, K. W. and Huang, A. and Neofotis, P. and McKie-Krisberg, Z.},
abstractNote = {ABSTRACT The green alga Scenedesmus obliquus is an emerging platform species for the industrial production of biofuels. Here, we report the draft assembly and annotation for the nuclear, plastid, and mitochondrial genomes of S. obliquus strain DOE0152z.},
doi = {10.1128/genomeA.00617-17},
journal = {Genome Announcements},
number = 32,
volume = 5,
place = {United States},
year = {Thu Aug 10 00:00:00 EDT 2017},
month = {Thu Aug 10 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
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  • Two-dimensional {sup 1}H NMR methods have been used to make sequence-specific resonance assignments for the 97 amino acid residues of the plastocyanin from the green alga Scenedesmus obliquus. Assignments were obtained for all backbone protons and the majority of the side-chain protons. Spin system identification relied heavily on the observation of relayed connectivities to the backbone amide proton. Sequence-specific assignments were made by using the sequential assignment procedure. During this process, an extra valine residue was identified that had not been detected in the original amino acid sequence. Elements of regular secondary structure were identified from characteristic NOE connectivities betweenmore » backbone protons, coupling constant values, and the observation of slowly exchanging amide protons. The protein in solution contains eight {beta}-strands, one short segment of helix, five reverse turns, and five loops. The {beta}-strands may be arranged into two {beta}sheets on the basis of extensive cross-strand NOE connectivities. The chain-folding topology determined from the NMR experiments is that of a Greek key {beta}-barrel and is similar to that observed for French bean plastocyanin in solution and poplar plastocyanin in the crystalline state. While the overall structures are similar, several differences in local structure between the S. obliquus and higher plant plastocyanins have been identified.« less
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