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Title: Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection

Authors:
 [1]; ORCiD logo [2];  [3];  [1];  [4];  [5];  [1];  [1];  [6];  [1];  [5];  [6];  [7];  [1]; ORCiD logo [8]
  1. USDA ARS Western Regional Research Center, 800 Buchanan St. Albany CA 94710-1105 USA
  2. Genomic Analysis Laboratory, The Salk Institute for Biological Studies and the Howard Hughes Medical Institute, 10010 North Torrey Pines Rd. La Jolla CA 92037 USA, DOE Joint Genome Institute, 2800 Mitchell Dr. Walnut Creek CA 94598 USA
  3. USDA ARS Western Regional Research Center, 800 Buchanan St. Albany CA 94710-1105 USA, DOE Joint Genome Institute, 2800 Mitchell Dr. Walnut Creek CA 94598 USA
  4. DOE Joint Genome Institute, 2800 Mitchell Dr. Walnut Creek CA 94598 USA
  5. Cereal Research Centre, Agriculture and Agri-Food Canada, 101 Route 100, Unit 100 Morden MB R6M 1Y5 Canada
  6. Department of Plant and Microbial Biology, University of California, 111 Koshland Hall Berkeley CA 94720 USA
  7. Genomic Analysis Laboratory, The Salk Institute for Biological Studies and the Howard Hughes Medical Institute, 10010 North Torrey Pines Rd. La Jolla CA 92037 USA
  8. USDA ARS Western Regional Research Center, 800 Buchanan St. Albany CA 94710-1105 USA, DOE Joint Genome Institute, 2800 Mitchell Dr. Walnut Creek CA 94598 USA, Department of Plant and Microbial Biology, University of California, 111 Koshland Hall Berkeley CA 94720 USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1401782
Grant/Contract Number:
SC0001526; AI02-07ER64452; AC02-05CH11231; SC0012400
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Volume: 91; Journal Issue: 3; Related Information: CHORUS Timestamp: 2017-10-20 17:28:13; Journal ID: ISSN 0960-7412
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Hsia, Mon Mandy, O'Malley, Ronan, Cartwright, Amy, Nieu, Rita, Gordon, Sean P., Kelly, Sandra, Williams, Tina G., Wood, Delilah F., Zhao, Yunjun, Bragg, Jennifer, Jordan, Mark, Pauly, Markus, Ecker, Joseph R., Gu, Yong, and Vogel, John P.. Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection. United Kingdom: N. p., 2017. Web. doi:10.1111/tpj.13582.
Hsia, Mon Mandy, O'Malley, Ronan, Cartwright, Amy, Nieu, Rita, Gordon, Sean P., Kelly, Sandra, Williams, Tina G., Wood, Delilah F., Zhao, Yunjun, Bragg, Jennifer, Jordan, Mark, Pauly, Markus, Ecker, Joseph R., Gu, Yong, & Vogel, John P.. Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection. United Kingdom. doi:10.1111/tpj.13582.
Hsia, Mon Mandy, O'Malley, Ronan, Cartwright, Amy, Nieu, Rita, Gordon, Sean P., Kelly, Sandra, Williams, Tina G., Wood, Delilah F., Zhao, Yunjun, Bragg, Jennifer, Jordan, Mark, Pauly, Markus, Ecker, Joseph R., Gu, Yong, and Vogel, John P.. Wed . "Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection". United Kingdom. doi:10.1111/tpj.13582.
@article{osti_1401782,
title = {Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection},
author = {Hsia, Mon Mandy and O'Malley, Ronan and Cartwright, Amy and Nieu, Rita and Gordon, Sean P. and Kelly, Sandra and Williams, Tina G. and Wood, Delilah F. and Zhao, Yunjun and Bragg, Jennifer and Jordan, Mark and Pauly, Markus and Ecker, Joseph R. and Gu, Yong and Vogel, John P.},
abstractNote = {},
doi = {10.1111/tpj.13582},
journal = {The Plant Journal},
number = 3,
volume = 91,
place = {United Kingdom},
year = {Wed Jun 21 00:00:00 EDT 2017},
month = {Wed Jun 21 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on June 21, 2018
Publisher's Accepted Manuscript

Citation Metrics:
Cited by: 2works
Citation information provided by
Web of Science

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  • In this study, the genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brachypodium has relied mainly on Agrobacterium tumefaciens strain AGL1. Currently the antibiotic hygromycin B has been the selective agent of choice for robust identification of transgenic calli in Brachypodium distachyon and Brachypodium sylvaticum but few other chemicals have been shown to work as well for selection of transgenic Brachypodium cells in tissue culture. This studymore » demonstrates that Agrobacterium rhizogenes strain 18r12v and paromomycin selection can be successfully used for the efficient generation of transgenic B. distachyon and B. sylvaticurn. Additionally we observed that the transformation rates were similar to or higher than those obtained with A. turnefaciens strain AGL1 and hygromycin selection. The A. rhizogenes strain 18r12v harboring the pARS1 binary vector and paromomycin selection is an effective means of generating transgenic Brachypodium plants. This novel approach will facilitate the transgenic complementation of T-DNA knockout mutants of B. distachyon which were created using hygromycin selection, as well as aid the implementation of more complex genome manipulation strategies which require multiple rounds of transformation.« less
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