There and Back Again: Loss and Reacquisition of Two‐Cys Photocycles in Cyanobacteriochromes

Rockwell, Nathan C.; Martin, Shelley S.; Lagarias, John Clark

doi:10.1111/php.12708

Title: There and Back Again: Loss and Reacquisition of Two‐Cys Photocycles in Cyanobacteriochromes

Journal Article · Sat May 13 00:00:00 EDT 2017 · Photochemistry and Photobiology

DOI:https://doi.org/10.1111/php.12708· OSTI ID:1401740

Rockwell, Nathan C. ^[1]; Martin, Shelley S. ^[1]; Lagarias, John Clark ^[1]

Department of Molecular and Cellular Biology University of California Davis CA

Abstract Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Both families use linear tetrapyrrole (bilin) chromophores that are covalently attached to a conserved Cys residue. CBCRs are more spectrally diverse than phytochromes, with known examples detecting light from the near ultraviolet to the edge of the infrared (370‐750 nm). Detection of ultraviolet to blue light by CBCRs is mediated by a second Cys residue, which forms a covalent linkage to the bilin C10 atom. Second linkage formation is best understood in a subfamily possessing a conserved Asp‐Xaa‐Cys‐Phe (DXCF) motif. Some DXCF CBCRs can isomerize their phycocyanobilin (PCB) chromophores into phycoviolobilin (PVB), a property not reported for other lineages. Both the DXCF Cys and PVB formation have been lost during evolution of other CBCR subfamilies. Using phylogenetic analysis and characterization of recombinantly expressed CBCRs, we show that the DXCF Cys residue has also been reacquired during CBCR evolution. Guided by this knowledge, we successfully reintroduced a second cysteine into a red/green CBCR, restoring blue‐light sensing and PVB formation with two additional substitutions. Our results validate the roles of these residues in CBCR spectral tuning and thus provide new insight into the molecular basis of their spectral diversity.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: DOE DE‐SC0002395

OSTI ID:: 1401740

Journal Information:: Photochemistry and Photobiology, Journal Name: Photochemistry and Photobiology Vol. 93 Journal Issue: 3; ISSN 0031-8655

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 25 works

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