L isteria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c‐di‐ GMP ‐dependent regulation

Köseoğlu, Volkan K.; Heiss, Christian; Azadi, Parastoo; Topchiy, Elena; Güvener, Zehra T.; Lehmann, Teresa E.; Miller, Kurt W.; Gomelsky, Mark

doi:10.1111/mmi.12966

Title: L isteria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c‐di‐ GMP ‐dependent regulation

Journal Article · Wed Mar 11 00:00:00 EDT 2015 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.12966· OSTI ID:1401610

Köseoğlu, Volkan K. ^[1]; Heiss, Christian ^[2]; Azadi, Parastoo ^[2]; Topchiy, Elena ^[3]; Güvener, Zehra T. ^[1]; Lehmann, Teresa E. ^[3]; Miller, Kurt W. ^[1]; Gomelsky, Mark ^[1]

Department of Molecular Biology University of Wyoming Laramie WY 82071 USA
Complex Carbohydrate Research Center University of Georgia Athens Georgia 30602 USA
Department of Chemistry University of Wyoming Laramie WY 82071 USA

Summary Elevated levels of the second messenger c‐di‐ GMP activate biosynthesis of an unknown exopolysaccharide ( EPS ) in the food‐borne pathogen L isteria monocytogenes . This EPS strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety. We analyzed the potential phylogenetic origin of this EPS , determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and identified diguanylate cyclases activating its synthesis. Phylogenetic analysis of EPS biosynthesis proteins suggests that they have evolved within monoderms. Scanning electron microscopy revealed that L . monocytogenes EPS is cell surface‐bound. Secreted carbohydrates represent exclusively cell‐wall debris. Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β‐1,4‐linked N ‐acetylmannosamine chain decorated with terminal α‐1,6‐linked galactose. All genes of the pss A ‐ E operon are required for EPS production and so is a separately located pss Z gene. We show that P ss Z has an EPS ‐specific glycosylhydrolase activity. Exogenously added P ss Z prevents EPS ‐mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active. The diguanylate cyclases D gc A and D gc B , whose genes are located next to pss Z , are primarily responsible for c‐di‐ GMP ‐dependent EPS production.

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Sponsoring Organization:: USDOE

OSTI ID:: 1401610

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Vol. 96 Journal Issue: 4; ISSN 0950-382X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: FAO

Language:: English

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