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Title: Enhanced production of 2,3-butanediol in pyruvate decarboxylase-deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose

Authors:
 [1];  [1];  [1];  [2];  [2];  [3];  [2];  [1]
  1. Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul Republic of Korea
  2. Department of Food Science and Human Nutrition, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana Illinois USA
  3. Department of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul Republic of Korea
Publication Date:
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1401461
Grant/Contract Number:
2011-0031359
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Biotechnology Journal
Additional Journal Information:
Journal Volume: 11; Journal Issue: 11; Related Information: CHORUS Timestamp: 2017-10-20 17:24:35; Journal ID: ISSN 1860-6768
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Choi, Eun-Ji, Kim, Jin-Woo, Kim, Soo-Jung, Seo, Seung-Oh, Lane, Stephan, Park, Yong-Cheol, Jin, Yong-Su, and Seo, Jin-Ho. Enhanced production of 2,3-butanediol in pyruvate decarboxylase-deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose. Country unknown/Code not available: N. p., 2016. Web. doi:10.1002/biot.201600042.
Choi, Eun-Ji, Kim, Jin-Woo, Kim, Soo-Jung, Seo, Seung-Oh, Lane, Stephan, Park, Yong-Cheol, Jin, Yong-Su, & Seo, Jin-Ho. Enhanced production of 2,3-butanediol in pyruvate decarboxylase-deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose. Country unknown/Code not available. doi:10.1002/biot.201600042.
Choi, Eun-Ji, Kim, Jin-Woo, Kim, Soo-Jung, Seo, Seung-Oh, Lane, Stephan, Park, Yong-Cheol, Jin, Yong-Su, and Seo, Jin-Ho. Mon . "Enhanced production of 2,3-butanediol in pyruvate decarboxylase-deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose". Country unknown/Code not available. doi:10.1002/biot.201600042.
@article{osti_1401461,
title = {Enhanced production of 2,3-butanediol in pyruvate decarboxylase-deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose},
author = {Choi, Eun-Ji and Kim, Jin-Woo and Kim, Soo-Jung and Seo, Seung-Oh and Lane, Stephan and Park, Yong-Cheol and Jin, Yong-Su and Seo, Jin-Ho},
abstractNote = {},
doi = {10.1002/biot.201600042},
journal = {Biotechnology Journal},
number = 11,
volume = 11,
place = {Country unknown/Code not available},
year = {Mon Sep 26 00:00:00 EDT 2016},
month = {Mon Sep 26 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1002/biot.201600042

Citation Metrics:
Cited by: 2works
Citation information provided by
Web of Science

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  • Pyruvate decarboxylase is a key enzyme in the production of low-molecular-weight byproducts (ethanol, acetate) in biomass-directed applications of Saccharomyces cerevisiae. To investigate whether decreased expression levels of pyruvate decarboxylase can reduce byproduct formation, the PDC2 gene, which encodes a positive regulator of pyruvate-decarboxylase synthesis, was inactivated in the prototrophic strain S. cerevisiae CEN.PK113-7D. This caused a 3--4-fold reduction of pyruvate-decarboxylase activity in glucose-limited, aerobic chemostat cultures grown at a dilution rate of 0.10 h{sup {minus}1}. Upon exposure of such cultures to a 50 mM glucose pulse, ethanol and acetate were the major byproducts formed by the wild type. In themore » pdc2{Delta} strain, formation of ethanol and acetate was reduced by 60--70%. In contrast to the wild type, the pdc2{Delta} strain produced substantial amounts of pyruvate after a glucose pulse. Nevertheless, its overall byproduct formation was ca. 50% lower. The specific rate of glucose consumption after a glucose pulse to pdc2{Delta} cultures was about 40% lower than in wild-type cultures.« less
  • 2,3-Butanediol is a feedstock chemical of potential industrial importance. It can serve as a monomer for many polymers of consumer interest that are currently supplied by the fossil fuel industry. Bacillus polymyxa can grow on inexpensive waste products of the food-processing industry and produce this glycol. This paper describes a mutant strain of B. polymyxa which displays constitutive production of catabolic ..cap alpha..-acetolactate synthase, an enzyme in the 2,3-butanediol pathway which is normally produced only in the late log or stationary phase of growth. The mutant was obtained by treating the wild type with nitrosoguanidine and subjecting it to amore » penicillin counterselection procedure. One of the selected mutant strains produced four times as much of the glycol as the wild type and utilized approximately 25% of the energy source, compared with essentially complete utilization of the energy source by the wild type. Studies are under way to optimize the production of the glycol by the mutant.« less
  • To develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production from biomass, we seek to redirect the carbon flow of the model ethanologen Zymomonas mobilis to produce desirable hydrocarbon intermediate 2,3-butanediol (2,3-BDO). 2,3-BDO is a bulk chemical building block, and can be upgraded in high yields to gasoline, diesel, and jet fuel. 2,3-BDO biosynthesis pathways from various bacterial species were examined, which include three genes encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. Bioinformatics analysis was carried out to pinpoint potential bottlenecks formore » high 2,3-BDO production. Different combinations of 2,3-BDO biosynthesis metabolic pathways using genes from different bacterial species have been constructed. Our results demonstrated that carbon flux can be deviated from ethanol production into 2,3-BDO biosynthesis, and all three heterologous genes are essential to efficiently redirect pyruvate from ethanol production for high 2,3-BDO production in Z. mobilis. The down-selection of best gene combinations up to now enabled Z. mobilis to reach the 2,3-BDO production of more than 10 g/L from glucose and xylose, as well as mixed C6/C5 sugar streams derived from the deacetylation and mechanical refining process. In conclusion, this study confirms the value of integrating bioinformatics analysis and systems biology data during metabolic engineering endeavors, provides guidance for value-added chemical production in Z. mobilis, and reveals the interactions between host metabolism, oxygen levels, and a heterologous 2,3-BDO biosynthesis pathway. Taken together, this work provides guidance for future metabolic engineering efforts aimed at boosting 2,3-BDO titer anaerobically.« less
  • Production of 2,3-butanediol by Klebsiella oxytoca is influenced by the degree of oxygen limitation. During batch culture studies, two phases of growth are observed: energy-coupled growth, during which cell growth and oxygen supply are coupled; and, energy-uncoupled growth, which arises when the degree of oxygen limitation reaches a critical value. Optimal 2,3-butanediol productivity occurs during the energy-coupled growth phase. In this article, a control system which maintains the batch culture at a constant level of oxygen limitation in the energy-coupled growth regime has been designed. Control, which involves feedback control on the oxygen transfer coefficient, is achieved by continually increasingmore » the partial pressure of oxygen in the feed gas, which in turn continually increases the oxygen transfer rate. Control has resulted in a balanced state of growth, a repression of ethanol formation, and an increase in 2,3-butanediol productivity of 18%.« less