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Title: Enhanced production of 2,3‐butanediol in pyruvate decarboxylase‐deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose

Journal Article · · Biotechnology Journal
 [1];  [1];  [1];  [2];  [2];  [3];  [2];  [1]
  1. Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University Seoul Republic of Korea
  2. Department of Food Science and Human Nutrition, and Institute for Genomic Biology, University of Illinois at Urbana‐Champaign Urbana Illinois USA
  3. Department of Advanced Fermentation Fusion Science and Technology, Kookmin University Seoul Republic of Korea
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Abstract Galactose and glucose are two of the most abundant monomeric sugars in hydrolysates of marine biomasses. While Saccharomyces cerevisiae can ferment galactose, its uptake is tightly controlled in the presence of glucose by catabolite repression. It is desirable to construct engineered strains capable of simultaneous utilization of glucose and galactose for producing biofuels and chemicals from marine biomass. The MTH1 gene coding for transcription factor in glucose signaling was mutated in a pyruvate decarboxylase (Pdc)‐deficient S. cerevisiae expressing heterologous 2,3‐butanediol (2,3‐BD) biosynthetic genes. The engineered S. cerevisiae strain consumed glucose and galactose simultaneously and produced 2,3‐BD as a major product. Total sugar consumption rates increased with a low ratio of glucose/galactose, though, occurrence of the glucose depletion in a fed‐batch fermentation decreased 2,3‐BD production substantially. Through optimizing the profiles of sugar concentrations in a fed‐batch cultivation with the engineered strain, 99.1 ± 1.7 g/L 2,3‐BD was produced in 143 hours with a yield of 0.353 ± 0.022 g 2,3‐BD/g sugars. This result suggests that simultaneous and efficient utilization of glucose and galactose by the engineered yeast might be applicable to the economical production of not only 2,3‐BD, but also other biofuels and chemicals from marine biomass.

Sponsoring Organization:
USDOE
OSTI ID:
1401461
Journal Information:
Biotechnology Journal, Journal Name: Biotechnology Journal Vol. 11 Journal Issue: 11; ISSN 1860-6768
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
Germany
Language:
English
Citation Metrics:
Cited by: 12 works
Citation information provided by
Web of Science

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