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Title: Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids

Abstract

ABSTRACT Monoterpenes (C 10 isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C 15 products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli 's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8‐cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high‐value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703–1712. © 2017 Wiley Periodicals, Inc.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]; ORCiD logo [1]
  1. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Technical Univ. of Denmark, Horsholm (Denmark); Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1379914
Alternate Identifier(s):
OSTI ID: 1401291
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 114; Journal Issue: 8; Journal ID: ISSN 0006-3592
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mendez-Perez, Daniel, Alonso-Gutierrez, Jorge, Hu, Qijun, Molinas, Margaux, Baidoo, Edward E. K., Wang, George, Chan, Leanne J. G., Adams, Paul D., Petzold, Christopher J., Keasling, Jay D., and Lee, Taek S. Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids. United States: N. p., 2017. Web. doi:10.1002/bit.26296.
Mendez-Perez, Daniel, Alonso-Gutierrez, Jorge, Hu, Qijun, Molinas, Margaux, Baidoo, Edward E. K., Wang, George, Chan, Leanne J. G., Adams, Paul D., Petzold, Christopher J., Keasling, Jay D., & Lee, Taek S. Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids. United States. https://doi.org/10.1002/bit.26296
Mendez-Perez, Daniel, Alonso-Gutierrez, Jorge, Hu, Qijun, Molinas, Margaux, Baidoo, Edward E. K., Wang, George, Chan, Leanne J. G., Adams, Paul D., Petzold, Christopher J., Keasling, Jay D., and Lee, Taek S. 2017. "Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids". United States. https://doi.org/10.1002/bit.26296. https://www.osti.gov/servlets/purl/1379914.
@article{osti_1379914,
title = {Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids},
author = {Mendez-Perez, Daniel and Alonso-Gutierrez, Jorge and Hu, Qijun and Molinas, Margaux and Baidoo, Edward E. K. and Wang, George and Chan, Leanne J. G. and Adams, Paul D. and Petzold, Christopher J. and Keasling, Jay D. and Lee, Taek S.},
abstractNote = {ABSTRACT Monoterpenes (C 10 isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C 15 products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli 's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8‐cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high‐value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703–1712. © 2017 Wiley Periodicals, Inc.},
doi = {10.1002/bit.26296},
url = {https://www.osti.gov/biblio/1379914}, journal = {Biotechnology and Bioengineering},
issn = {0006-3592},
number = 8,
volume = 114,
place = {United States},
year = {Thu May 18 00:00:00 EDT 2017},
month = {Thu May 18 00:00:00 EDT 2017}
}

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Cited by: 54 works
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Works referenced in this record:

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Works referencing / citing this record:

Taming the Reactivity of Monoterpene Synthases To Guide Regioselective Product Hydroxylation
journal, April 2020


Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate
journal, August 2019


Microbial synthesis of medium-chain chemicals from renewables
journal, December 2017


Microbial Platform for Terpenoid Production: Escherichia coli and Yeast
journal, October 2018


CO 2 to Terpenes: Autotrophic and Electroautotrophic α-Humulene Production with Cupriavidus necator
journal, January 2018


A Novel Process for Cadaverine Bio-Production Using a Consortium of Two Engineered Escherichia coli
journal, June 2018


Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
journal, October 2018


CO 2 to Terpenes: Autotrophic and Electroautotrophic α-Humulene Production with Cupriavidus necator
journal, January 2018


Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate
journal, August 2019


Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
journal, October 2018


A mevalonate bypass system facilitates elucidation of plastid biology in malaria parasites
journal, February 2020


Microbial Platform for Terpenoid Production: Escherichia coli and Yeast
journal, October 2018