Engineering the iron‐oxidizing chemolithoautotroph Acidithiobacillus ferrooxidans for biochemical production

Kernan, Timothy; Majumdar, Sudipta; Li, Xiaozheng; Guan, Jingyang; West, Alan C.; Banta, Scott

doi:10.1002/bit.25703

Title: Engineering the iron‐oxidizing chemolithoautotroph Acidithiobacillus ferrooxidans for biochemical production

Journal Article · Tue Oct 20 00:00:00 EDT 2015 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.25703· OSTI ID:1401091

Kernan, Timothy ^[1]; Majumdar, Sudipta ^[1]; Li, Xiaozheng ^[1]; Guan, Jingyang ^[1]; West, Alan C. ^[1]; Banta, Scott ^[1]

Department of Chemical Engineering Columbia University 500 W. 120th Street New York City New York 10027

ABSTRACT There is growing interest in developing non‐photosynthetic routes for the conversion of CO ₂ to fuels and chemicals. One underexplored approach is the transfer of energy to the metabolism of genetically modified chemolithoautotrophic bacteria. Acidithiobacillus ferrooxidans is an obligate chemolithoautotroph that derives its metabolic energy from the oxidation of iron or sulfur at low pH. Two heterologous biosynthetic pathways have been expressed in A. ferrooxidans to produce either isobutyric acid or heptadecane from CO ₂ and the oxidation of Fe ²⁺ . A sevenfold improvement in productivity of isobutyric acid was obtained through improved media formulations in batch cultures. Steady‐state efficiencies were lower in continuous cultures, likely due to ferric inhibition. If coupled to solar panels, the photon‐to‐fuel efficiency of this proof‐of‐principle process approaches estimates for agriculture‐derived biofuels. These efforts lay the foundation for the utilization of this organism in the exploitation of electrical energy for biochemical synthesis. Biotechnol. Bioeng. 2016;113: 189–197. © 2015 Wiley Periodicals, Inc.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: DE‐AR0000060

OSTI ID:: 1401091

Journal Information:: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Vol. 113 Journal Issue: 1; ISSN 0006-3592

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 30 works

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