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Title: Photosynthetic terpene hydrocarbon production for fuels and chemicals

 [1];  [2];  [1]
  1. Department of Plant Pathology and Microbiology, Texas A&M University, College Station TX USA, Synthetic and Systems Biology Innovation Hub, Texas A&M University, College Station TX USA, Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station TX USA
  2. Global Change and Photosynthesis Research Unit, USDA/ARS, Urbana IL USA, Institute for Genomic Biology, University of Illinois, Urbana IL USA
Publication Date:
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 13; Journal Issue: 2; Related Information: CHORUS Timestamp: 2017-10-20 16:21:16; Journal ID: ISSN 1467-7644
Country of Publication:
United Kingdom

Citation Formats

Wang, Xin, Ort, Donald R., and Yuan, Joshua S.. Photosynthetic terpene hydrocarbon production for fuels and chemicals. United Kingdom: N. p., 2015. Web. doi:10.1111/pbi.12343.
Wang, Xin, Ort, Donald R., & Yuan, Joshua S.. Photosynthetic terpene hydrocarbon production for fuels and chemicals. United Kingdom. doi:10.1111/pbi.12343.
Wang, Xin, Ort, Donald R., and Yuan, Joshua S.. 2015. "Photosynthetic terpene hydrocarbon production for fuels and chemicals". United Kingdom. doi:10.1111/pbi.12343.
title = {Photosynthetic terpene hydrocarbon production for fuels and chemicals},
author = {Wang, Xin and Ort, Donald R. and Yuan, Joshua S.},
abstractNote = {},
doi = {10.1111/pbi.12343},
journal = {Plant Biotechnology Journal},
number = 2,
volume = 13,
place = {United Kingdom},
year = 2015,
month = 1

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1111/pbi.12343

Citation Metrics:
Cited by: 14works
Citation information provided by
Web of Science

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  • Photosynthetic hydrocarbon production bypasses the traditional biomass hydrolysis process and represents the most direct conversion of sunlight energy into the next-generation biofuels. As a major class of biologically derived hydrocarbons with diverse structures, terpenes are also valuable in producing a variety of fungible bioproducts in addition to the advanced drop-in' biofuels. However, it is highly challenging to achieve the efficient redirection of photosynthetic carbon and reductant into terpene biosynthesis. In this review, we discuss four major scientific and technical barriers for photosynthetic terpene production and recent advances to address these constraints. Collectively, photosynthetic terpene production needs to be optimized inmore » a systematic fashion, in which the photosynthesis improvement, the optimization of terpene biosynthesis pathway, the improvement of key enzymes and the enhancement of sink effect through terpene storage or secretion are all important. New advances in synthetic biology also offer a suite of potential tools to design and engineer photosynthetic terpene platforms. The systemic integration of these solutions may lead to disruptive' technologies to enable biofuels and bioproducts with high efficiency, yield and infrastructure compatibility.« less
  • When sweet potato (Ipomoea batatas) root tissue was infected by Ceratocystis fimbriata, activity of the enzyme system from mevalonate to isopentenyl pyrophosphate, especially of pyrophosphomevalonate decarboxylase (EC, was increased in the noninfected tissue adjacent to the infected region, preceding the furano-terpene production in the infected region. Cutting and incubation of sweet potato slices did not produce furano-terpenes, and only slightly increased the activity of the enzyme system from mevalonate to isopentenyl pyrophosphate. The enzymic activity in diseased tissue was localized in the soluble fraction, and was higher in the tissue from the surface to a depth of about 5more » mm with gradual decrease toward the inner part. Mercuric chloride (0.1%, w/v) and sodium dodecyl sulfate (1.0%, w/v) were utilized as model inducers of furano-terpenes and pyrophosphomevalonate decarboxylase. The mercuric chloride- or sodium dodecyl sulfate-induced response was inhibited by administration of cycloheximide to the discs together with the inducer immediately after disc preparation. When cycloheximide or blasticidin S was applied together with the inducer, to the discs 9 hours or more after disc preparation, the induction was not inhibited but rather stimulated.« less
  • By inserting metallocatalysts (such as platinum, osmium, or ruthenium) at the reducing site of photosystem I (PSI), electrons that emerge from PSI can be channeled to various redox reactions that could potentially produce fuels and chemicals (such as H{sub 2} and CH{sub 4}, and so forth) instead of NADP{sup +} reduction, as in natural photosynthesis. We have recently developed a technique to photoprecipitate metallocatalysts in situ at the reducing site of PSI in thylakoid membranes, using water-soluble hexachloroplatinate, hexachloroosmiate, and hexachlororuthenate at biological temperature and neutral pH. This technique combined with {open_quotes}rewiring{close_quotes} of photosynthesis is a potentially important new fieldmore » of biometallocatalysis. Potential applications of biometallocatalysis will be addressed in this article.« less