Expression activation and functional analysis of HLA 3, a putative inorganic carbon transporter in C hlamydomonas reinhardtii

Gao, Han; Wang, Yingjun; Fei, Xiaowen; Wright, David A.; Spalding, Martin H.

doi:10.1111/tpj.12788

Title: Expression activation and functional analysis of HLA 3, a putative inorganic carbon transporter in C hlamydomonas reinhardtii

Journal Article · Tue Mar 24 00:00:00 EDT 2015 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.12788· OSTI ID:1400690

Gao, Han ^[1]; Wang, Yingjun ^[1]; Fei, Xiaowen ^[1]; Wright, David A. ^[1]; Spalding, Martin H. ^[1]

Department of Genetics, Development, and Cell Biology Iowa State University Ames IA 50011 USA

Summary The CO ₂ concentrating mechanism (CCM) is a key component of the carbon assimilation strategy of aquatic microalgae. Induced by limiting CO ₂ and tightly regulated, the CCM enables these microalgae to respond rapidly to varying environmental CO ₂ supplies and to perform photosynthetic CO ₂ assimilation in a cost‐effective way. A functional CCM in eukaryotic algae requires Rubisco sequestration, rapid interconversion between CO ₂ and catalyzed by carbonic anhydrases (CAs), and active inorganic carbon (Ci) uptake. In the model microalga Chlamydomonas reinhardtii , a membrane protein HLA3 is proposed to be involved in active Ci uptake across the plasma membrane. In this study, we use an artificially designed transcription activator‐like effector ( dTALE ) to activate the expression of HLA3. The successful activation of HLA3 expression demonstrates dTALE as a promising tool for gene‐specific activation and investigation of gene function in Chlamydomonas . Activation of HLA3 expression in high CO ₂ acclimated cells, where HLA3 is not expressed, resulted in increased Ci accumulation and Ci‐dependent photosynthetic O ₂ evolution specifically in very low CO ₂ concentrations, which confirms that HLA3 is indeed involved in Ci uptake, and suggests it is mainly associated with transport in very low CO ₂ concentrations, conditions in which active CO ₂ uptake is highly limited.

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Sponsoring Organization:: USDOE

OSTI ID:: 1400690

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Vol. 82 Journal Issue: 1; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 45 works

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