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Title: Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin

Authors:
 [1];  [1];  [2];  [2];  [3];  [4];  [5];  [2]
  1. Biology of Reproduction and Stem Cell Group, Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra Portugal
  2. Biology of Reproduction and Stem Cell Group, Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra Portugal, Institute for Interdisciplinary Research (IIIUC), University of Coimbra, Coimbra Portugal
  3. Institute for Interdisciplinary Research (IIIUC), University of Coimbra, Coimbra Portugal, PhD Program in Experimental Biology and Biomedicine (PDBEB), Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra Portugal
  4. Centre of Reproductive Medicine and Andrology, Institute of Reproductive and Regenerative Biology, University of Münster, Münster Germany
  5. Biology of Reproduction and Stem Cell Group, Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra Portugal, Department of Life Sciences, University of Coimbra, Coimbra Portugal
Publication Date:
Sponsoring Org.:
USDOE Office of Nuclear Energy (NE), Fuel Cycle Technologies (NE-5)
OSTI Identifier:
1400565
Grant/Contract Number:
SFRH/BPD/74252/2010; Pest-C/SAU/LA0001/2013-2014
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Andrology
Additional Journal Information:
Journal Volume: 4; Journal Issue: 6; Related Information: CHORUS Timestamp: 2017-10-20 15:02:39; Journal ID: ISSN 2047-2919
Publisher:
Wiley-Blackwell
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Escada-Rebelo, S., Silva, A. F., Amaral, S., Tavares, R. S., Paiva, C., Schlatt, S., Ramalho-Santos, J., and Mota, P. C. Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin. Country unknown/Code not available: N. p., 2016. Web. doi:10.1111/andr.12223.
Escada-Rebelo, S., Silva, A. F., Amaral, S., Tavares, R. S., Paiva, C., Schlatt, S., Ramalho-Santos, J., & Mota, P. C. Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin. Country unknown/Code not available. doi:10.1111/andr.12223.
Escada-Rebelo, S., Silva, A. F., Amaral, S., Tavares, R. S., Paiva, C., Schlatt, S., Ramalho-Santos, J., and Mota, P. C. Fri . "Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin". Country unknown/Code not available. doi:10.1111/andr.12223.
@article{osti_1400565,
title = {Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin},
author = {Escada-Rebelo, S. and Silva, A. F. and Amaral, S. and Tavares, R. S. and Paiva, C. and Schlatt, S. and Ramalho-Santos, J. and Mota, P. C.},
abstractNote = {},
doi = {10.1111/andr.12223},
journal = {Andrology},
number = 6,
volume = 4,
place = {Country unknown/Code not available},
year = {Fri Jun 17 00:00:00 EDT 2016},
month = {Fri Jun 17 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1111/andr.12223

Citation Metrics:
Cited by: 1work
Citation information provided by
Web of Science

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  • After 1000 r of acute localized irradiation many spermatocytes are blocked in first metaphase. Many restitution nuclei similar to those seen after colchicine treatment form between the first and the tenth day after irradiation, the maximum being observed at 6 days. These restituted cells often drift toward the basement membrane. In agreement with previous investigators we have seen an excess number of Sertoli cells in heavily damaged tubules. This excess is not explained by cell division as shown by autoradiography, nor by shrinkage of tubules, since the number is abnormally high even after correction for the shrinkage. A major partmore » of this excess apparently corresponds to the number of restitution cells formed from the blocked first metaphases. The ultimate fate of these indifferent "Sertoli"-like cells is unknown. We have not observed them entering a new spermatogenic cycle. Type A sper- matogonia were observed between 8 and 10 days after 1000 r with a frequency of 0.007 per tubule. After division, the resulting daughter cells distribute themselves along the basement membrane, soon forming a complete ring of dividing spermatogenic cells, observed at 20 days in recovering tubules. Stage "XII" was observed at 5 weeks showing development only through first metaphase spermatocytes. The present findings appear to explain the discrepancies introduced by workers in the 1920's and not resolved since.« less
  • The effect of single doses of vincristine (VCR) or bleomycin (BLM) on mice spermatogonia was investigated, and the influence of either of these drugs on the radiation response of murine spermatogonial stem cells was examined. When assessed by flow cytometry, VCR (1.0 mg/kg) or BLM (100 mg/kg) reduced the survival in the differentiated spermatogonia to 4% and 37% of controls, respectively (p less than 0.05). VCR reduced the stem cells to 79% of controls (p less than 0.05), whereas BLM had no apparent effect on the stem cells (p greater than 0.05). Drugs were administered intraperitoneally up to 28 daysmore » before or after local irradiation with 9 Gy. VCR produced significant enhancement of radiation-induced damage to spermatogonial stem cells, which was most prominent when administered 6 or 12 hr after irradiation. BLM administered before irradiation or 1 hr after radiotherapy produced significant enhancement.« less
  • Dose-response studies of the radiosensitivity of spermatogonial stem cells in various epithelial stages after irradiation with graded doses of fission neutrons of 1 MeV mean energy were carried out in the Cpb-N mouse. These studies on the stem cell population in stages IX-XI yielded simple exponential lines characterized by an average D0 value of 0.76 +/- 0.02 Gy. In the subsequent epithelial stages XII-III, a significantly lower D0 value of 0.55 +/- 0.02 Gy was found. In contrast to the curves obtained for stem cells in stages IX-III, the curves obtained in stages IV-VIII indicated the presence of a mixturemore » of radioresistant and radiosensitive stem cells. In stage VII, almost no radioresistant stem cells appeared to be present and a D0 value for the radiosensitive stem cells of 0.22 +/- 0.01 Gy was derived. Previously, data were obtained on the size of colonies (in number of spermatogonia) derived from surviving stem cells. Combining these data with data from the newly obtained dose-response curves yielded the number of stem cells, per stage and with the specific radiosensitivities, present in the control epithelium. In stages IX-XI, there are approximately 6 stem cells per 1000 Sertoli cells with a radiosensitivity characterized by a D0 of 0.76 Gy, which corresponds to one-third of the As population in these stages. (The As spermatogonia are presumed to be the stem cells of spermatogenesis.) IN stages XII-III, there are approximately 12 stem cells per 1000 Sertoli cells with a radiosensitivity characterized by a D0 of 0.55 Gy, which roughly equals the number of A single spermatogonia in these stages. These calculations could not be made for stages IV-VIII since no simple exponential lines were obtained for these stages.« less