skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer

Abstract

Chromatin remodelers catalyze dynamic packaging of the genome by carrying out nucleosome assembly/disassembly, histone exchange, and nucleosome repositioning. Remodeling results in evenly spaced nucleosomes, which requires probing both sides of the nucleosome, yet the way remodelers organize sliding activity to achieve this task is not understood. Here, we show that the monomeric Chd1 remodeler shifts DNA back and forth by dynamically alternating between different segments of the nucleosome. During sliding, Chd1 generates unstable remodeling intermediates that spontaneously relax to a pre-remodeled position. We demonstrate that nucleosome sliding is tightly controlled by two regulatory domains: the DNA-binding domain, which interferes with sliding when its range is limited by a truncated linking segment, and the chromodomains, which play a key role in substrate discrimination. We propose that active interplay of the ATPase motor with the regulatory domains may promote dynamic nucleosome structures uniquely suited for histone exchange and chromatin reorganization during transcription.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1400286
Resource Type:
Journal Article
Resource Relation:
Journal Name: Molecular Cell; Journal Volume: 68; Journal Issue: 1
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Qiu, Yupeng, Levendosky, Robert F., Chakravarthy, Srinivas, Patel, Ashok, Bowman, Gregory D., and Myong, Sua. The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer. United States: N. p., 2017. Web. doi:10.1016/j.molcel.2017.08.018.
Qiu, Yupeng, Levendosky, Robert F., Chakravarthy, Srinivas, Patel, Ashok, Bowman, Gregory D., & Myong, Sua. The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer. United States. doi:10.1016/j.molcel.2017.08.018.
Qiu, Yupeng, Levendosky, Robert F., Chakravarthy, Srinivas, Patel, Ashok, Bowman, Gregory D., and Myong, Sua. Sun . "The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer". United States. doi:10.1016/j.molcel.2017.08.018.
@article{osti_1400286,
title = {The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer},
author = {Qiu, Yupeng and Levendosky, Robert F. and Chakravarthy, Srinivas and Patel, Ashok and Bowman, Gregory D. and Myong, Sua},
abstractNote = {Chromatin remodelers catalyze dynamic packaging of the genome by carrying out nucleosome assembly/disassembly, histone exchange, and nucleosome repositioning. Remodeling results in evenly spaced nucleosomes, which requires probing both sides of the nucleosome, yet the way remodelers organize sliding activity to achieve this task is not understood. Here, we show that the monomeric Chd1 remodeler shifts DNA back and forth by dynamically alternating between different segments of the nucleosome. During sliding, Chd1 generates unstable remodeling intermediates that spontaneously relax to a pre-remodeled position. We demonstrate that nucleosome sliding is tightly controlled by two regulatory domains: the DNA-binding domain, which interferes with sliding when its range is limited by a truncated linking segment, and the chromodomains, which play a key role in substrate discrimination. We propose that active interplay of the ATPase motor with the regulatory domains may promote dynamic nucleosome structures uniquely suited for histone exchange and chromatin reorganization during transcription.},
doi = {10.1016/j.molcel.2017.08.018},
journal = {Molecular Cell},
number = 1,
volume = 68,
place = {United States},
year = {Sun Oct 01 00:00:00 EDT 2017},
month = {Sun Oct 01 00:00:00 EDT 2017}
}