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Title: Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates

Abstract

Metabolic synthesis of single cell oils (SCOs) for biodiesel application by heterotrophic oleaginous microorganisms is being hampered by the high cost of culture media. This study investigated the possibility of using loblolly pine and sweetgum autohydrolysates as economic feedstocks for microbial lipid production by oleaginous Rhodococcus opacus ( R. opacus) PD630 and DSM 1069. Results revealed that when the substrates were detoxified by the removal of inhibitors (such as HMF—hydroxymethyl-furfural), the two strains exhibited viable growth patterns after a short adaptation/lag phase. R. opacus PD630 accumulated as much as 28.6 % of its cell dry weight (CDW) in lipids while growing on detoxified sweetgum autohydrolysate (DSAH) that translates to 0.25 g/l lipid yield. The accumulation of SCOs reached the level of oleagenicity in DSM 1069 cells (28.3 % of CDW) as well, while being cultured on detoxified pine autohydrolysate (DPAH), with the maximum lipid yield of 0.31 g/l. The composition of the obtained microbial oils varied depending on the substrates provided. These results indicate that lignocellulosic autohydrolysates can be used as low-cost fermentation substrates for microbial lipid production by wild-type R. opacus species. Furthermore, the variety of applications for aqueous liquors from lignocellulosic pretreatment has been expanded, allowing for themore » further optimization of the integrated biorefinery.« less

Authors:
 [1];  [1];  [2];  [2];  [2];  [2];  [1]; ORCiD logo [3]
  1. Hunan Univ., Changsha (People's Republic of China)
  2. Georgia Inst. of Technology, Atlanta, GA (United States)
  3. Georgia Inst. of Technology, Atlanta, GA (United States); The Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1399965
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 99; Journal Issue: 17; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Autohydrolysis; Hardwood; Softwood; Fatty acid methyl ester; Oleaginous; Rhodococcus; Fermentation

Citation Formats

Wei, Zhen, Zeng, Guangming, Huang, Fang, Kosa, Matyas, Sun, Qining, Meng, Xianzhi, Huang, Danlian, and Ragauskas, Arthur J. Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates. United States: N. p., 2015. Web. doi:10.1007/s00253-015-6752-5.
Wei, Zhen, Zeng, Guangming, Huang, Fang, Kosa, Matyas, Sun, Qining, Meng, Xianzhi, Huang, Danlian, & Ragauskas, Arthur J. Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates. United States. doi:10.1007/s00253-015-6752-5.
Wei, Zhen, Zeng, Guangming, Huang, Fang, Kosa, Matyas, Sun, Qining, Meng, Xianzhi, Huang, Danlian, and Ragauskas, Arthur J. Sat . "Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates". United States. doi:10.1007/s00253-015-6752-5. https://www.osti.gov/servlets/purl/1399965.
@article{osti_1399965,
title = {Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates},
author = {Wei, Zhen and Zeng, Guangming and Huang, Fang and Kosa, Matyas and Sun, Qining and Meng, Xianzhi and Huang, Danlian and Ragauskas, Arthur J.},
abstractNote = {Metabolic synthesis of single cell oils (SCOs) for biodiesel application by heterotrophic oleaginous microorganisms is being hampered by the high cost of culture media. This study investigated the possibility of using loblolly pine and sweetgum autohydrolysates as economic feedstocks for microbial lipid production by oleaginous Rhodococcus opacus (R. opacus) PD630 and DSM 1069. Results revealed that when the substrates were detoxified by the removal of inhibitors (such as HMF—hydroxymethyl-furfural), the two strains exhibited viable growth patterns after a short adaptation/lag phase. R. opacus PD630 accumulated as much as 28.6 % of its cell dry weight (CDW) in lipids while growing on detoxified sweetgum autohydrolysate (DSAH) that translates to 0.25 g/l lipid yield. The accumulation of SCOs reached the level of oleagenicity in DSM 1069 cells (28.3 % of CDW) as well, while being cultured on detoxified pine autohydrolysate (DPAH), with the maximum lipid yield of 0.31 g/l. The composition of the obtained microbial oils varied depending on the substrates provided. These results indicate that lignocellulosic autohydrolysates can be used as low-cost fermentation substrates for microbial lipid production by wild-type R. opacus species. Furthermore, the variety of applications for aqueous liquors from lignocellulosic pretreatment has been expanded, allowing for the further optimization of the integrated biorefinery.},
doi = {10.1007/s00253-015-6752-5},
journal = {Applied Microbiology and Biotechnology},
number = 17,
volume = 99,
place = {United States},
year = {Sat Jul 04 00:00:00 EDT 2015},
month = {Sat Jul 04 00:00:00 EDT 2015}
}

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Works referenced in this record:

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journal, January 2007

  • Almeida, Joao; Modig, Tobias; Petersson, Anneli
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Perspectives of microbial oils for biodiesel production
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Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition
journal, August 2000


Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
journal, August 2000


Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010