A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Priye, Aashish; Bird, Sara W.; Light, Yooli K.; Ball, Cameron S.; Negrete, Oscar A.; Meagher, Robert J.

doi:10.1038/srep44778

Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Journal Article · Mon Mar 20 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/srep44778· OSTI ID:1399884

Priye, Aashish ^[1]; Bird, Sara W. ^[1]; Light, Yooli K. ^[2]; Ball, Cameron S. ^[1]; Negrete, Oscar A. ^[1]; Meagher, Robert J. ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biotechnology and Bioengineering
Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Systems Biology

Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. We demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. The smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Altogether, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.

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Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1399884

Report Number(s):: SAND2016-11988J; srep44778

Journal Information:: Scientific Reports, Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 191 works

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Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization Phan, Dinh-Tuan; Jin, Lin; Wustoni, Shofarul Lab on a Chip, Vol. 18, Issue 4 https://doi.org/10.1039/c7lc01066d	journal	January 2018
Multiplex sample-to-answer detection of bacteria using a pipette-actuated capillary array comb with integrated DNA extraction, isothermal amplification, and smartphone detection Hui, Junhou; Gu, Yin; Zhu, Yuanshou Lab on a Chip, Vol. 18, Issue 18 https://doi.org/10.1039/c8lc00543e	journal	January 2018
Digital polymerase chain reaction technology – recent advances and future perspectives Sreejith, Kamalalayam Rajan; Ooi, Chin Hong; Jin, Jing Lab on a Chip, Vol. 18, Issue 24 https://doi.org/10.1039/c8lc00990b	journal	January 2018
Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection Becherer, Lisa; Borst, Nadine; Bakheit, Mohammed Analytical Methods, Vol. 12, Issue 6 https://doi.org/10.1039/c9ay02246e	journal	January 2020
Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors Yang, Jiancheng; Carey, Patrick; Ren, Fan Applied Physics Letters, Vol. 113, Issue 3 https://doi.org/10.1063/1.5029902	journal	July 2018
Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) Castro, Talita; Sabalza, Maite; Barber, Cheryl Journal of Oral Microbiology, Vol. 10, Issue 1 https://doi.org/10.1080/20002297.2018.1510712	journal	January 2018
Innovative and New Approaches to Laboratory Diagnosis of Zika and Dengue: A Meeting Report Goncalves, Adriana; Peeling, Rosanna W.; Chu, May C. The Journal of Infectious Diseases, Vol. 217, Issue 7 https://doi.org/10.1093/infdis/jix678	journal	December 2017
Smartphone-based clinical diagnostics: towards democratization of evidence-based health care Hernández-Neuta, I.; Neumann, F.; Brightmeyer, J. Journal of Internal Medicine, Vol. 285, Issue 1 https://doi.org/10.1111/joim.12820	journal	September 2018
Mobile oral heath technologies based on saliva Meagher, Rj; Kousvelari, E. Oral Diseases, Vol. 24, Issue 1-2 https://doi.org/10.1111/odi.12775	journal	February 2018
Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus Natrajan, Muktha S.; Rojas, Alejandra; Waggoner, Jesse J. Journal of Clinical Microbiology, Vol. 57, Issue 6 https://doi.org/10.1128/jcm.00350-19	journal	May 2019
Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform Ahn, Su Jeong; Baek, Yun Hee; Lloren, Khristine Kaith S. BMC Infectious Diseases, Vol. 19, Issue 1 https://doi.org/10.1186/s12879-019-4277-8	journal	August 2019
Laboratory Diagnosis and Potential Application of Nucleic Acid Biosensor Approach for Early Detection of Dengue Virus Infections Izuan Abdul Rashid, Jahwarhar; Azah Yusof, Nor Biosciences, Biotechnology Research Asia, Vol. 15, Issue 2 https://doi.org/10.13005/bbra/2628	journal	June 2018
Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva Sabalza, Maite; Yasmin, Rubina; Barber, Cheryl A. PLOS ONE, Vol. 13, Issue 2 https://doi.org/10.1371/journal.pone.0192398	journal	February 2018
A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus Takemura, Kenshin; Adegoke, Oluwasesan; Suzuki, Tetsuro PLOS ONE, Vol. 14, Issue 1 https://doi.org/10.1371/journal.pone.0211517	journal	January 2019
Advances in Diagnosis, Surveillance, and Monitoring of Zika Virus: An Update Singh, Raj K.; Dhama, Kuldeep; Karthik, Kumaragurubaran Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.02677	journal	January 2018
Advances in Directly Amplifying Nucleic Acids from Complex Samples Walker, Faye M.; Hsieh, Kuangwen Biosensors, Vol. 9, Issue 4 https://doi.org/10.3390/bios9040117	journal	September 2019
Smartphone Coupled with a Paper-Based Colorimetric Device for Sensitive and Portable Mercury Ion Sensing Firdaus, M. Lutfi; Aprian, Angga; Meileza, Nessi Chemosensors, Vol. 7, Issue 2 https://doi.org/10.3390/chemosensors7020025	journal	May 2019
Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens Higgins, Owen; Clancy, Eoin; Cormican, Martin International Journal of Molecular Sciences, Vol. 19, Issue 2 https://doi.org/10.3390/ijms19020524	journal	February 2018
A Nontoxic Battery with 3D-Printed Housing for On-Demand Operation of Microcontrollers in Microfluidic Sensors Sachsenheimer, Kai; Richter, Christiane; Helmer, Dorothea Micromachines, Vol. 10, Issue 9 https://doi.org/10.3390/mi10090588	journal	September 2019
Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection Jiang, Xiao; Loeb, Julia C.; Manzanas, Carlos Angewandte Chemie, Vol. 130, Issue 52 https://doi.org/10.1002/ange.201809993	journal	November 2018
One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST) Berry, Scott M.; Alarid, Elaine T.; Beebe, David J. Lab on a Chip, Vol. 11, Issue 10, p. 1747-1753 https://doi.org/10.1039/c1lc00004g	journal	January 2011
DNA microarray analysis using a smartphone to detect the BRCA-1 gene Prasad, Alisha; Hasan, Syed Mohammad Abid; Grouchy, Steven The Analyst, Vol. 144, Issue 1 https://doi.org/10.1039/c8an01020j	journal	January 2019
An inexpensive smartphone-based device for point-of-care ovulation testing Potluri, Vaishnavi; Kathiresan, Preethi Sangeetha; Kandula, Hemanth Lab on a Chip, Vol. 19, Issue 1 https://doi.org/10.1039/c8lc00792f	journal	January 2019
Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection Velders, Aldrik H.; Schoen, Cor; Saggiomo, Vittorio BMC Research Notes, Vol. 11, Issue 1 https://doi.org/10.1186/s13104-018-3197-9	journal	February 2018
2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics Nguyen, Trieu; Duong Bang, Dang; Wolff, Anders Micromachines, Vol. 11, Issue 3 https://doi.org/10.3390/mi11030306	journal	March 2020
Developments in Transduction, Connectivity and AI/Machine Learning for Point-of-Care Testing O’Sullivan, Shane; Ali, Zulfiqur; Jiang, Xiaoyi Sensors, Vol. 19, Issue 8 https://doi.org/10.3390/s19081917	journal	April 2019
Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review Silva, Severino Jefferson Ribeiro da; Pardee, Keith; Pena, Lindomar Viruses, Vol. 12, Issue 1 https://doi.org/10.3390/v12010019	journal	December 2019
Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2 Baek, Yun Hee; Um, Jihye; Antigua, Khristine Joy C. Taylor & Francis https://doi.org/10.6084/m9.figshare.12154878	text	January 2020
Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP) Calvert, Amanda E.; Biggerstaff, Brad J.; Tanner, Nathan A. PLOS ONE, Vol. 12, Issue 9 https://doi.org/10.1371/journal.pone.0185340	journal	September 2017
Smartphone-based pathogen diagnosis in urinary sepsis patients Barnes, Lucien; Heithoff, Douglas M.; Mahan, Scott P. EBioMedicine, Vol. 36 https://doi.org/10.1016/j.ebiom.2018.09.001	journal	October 2018
A Rapid and Low-Cost Pathogen Detection Platform by Using a Molecular Agglutination Assay Wu, Tsung-Feng; Chen, Yu-Chen; Wang, Wei-Chung ACS Central Science, Vol. 4, Issue 11 https://doi.org/10.1021/acscentsci.8b00447	journal	November 2018
Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity Vietz, Carolin; Schütte, Max L.; Wei, Qingshan ACS Omega, Vol. 4, Issue 1 https://doi.org/10.1021/acsomega.8b03136	journal	January 2019
Smartphone‐based clinical diagnostics and atrial fibrillation: the need to avoid being out of sight Leggio, M.; Villano, A.; Fusco, A. Journal of Internal Medicine, Vol. 286, Issue 3 https://doi.org/10.1111/joim.12916	journal	May 2019
“The Smartphone’s Guide to the Galaxy”: In Situ Analysis in Space Nelis, Joost; Elliott, Christopher; Campbell, Katrina Biosensors, Vol. 8, Issue 4 https://doi.org/10.3390/bios8040096	journal	October 2018
Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening Yin, Kun; Pandian, Vikram; Kadimisetty, Karteek Theranostics, Vol. 9, Issue 9 https://doi.org/10.7150/thno.32224	journal	January 2019

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Additional file 1 of Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform Ahn, Su; Baek, Yun; Lloren, Khristine https://doi.org/10.6084/m9.figshare.9210812.v1 The current record is supplemented by this text	text	January 2019
Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform Ahn, Su; Baek, Yun; Lloren, Khristine https://doi.org/10.6084/m9.figshare.c.4604882.v1 The current record is supplemented by this collection	collection	January 2019