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Title: Real‐time crystallographic studies of the adenine riboswitch using an X‐ray free‐electron laser

Journal Article · · Federation of European Biochemical Societies (FEBS) Journal
DOI:https://doi.org/10.1111/febs.14110· OSTI ID:1399617
 [1];  [1];  [1];  [1];  [1]
  1. Structural Biophysics Laboratory National Cancer Institute Frederick MD USA
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Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X‐ray free‐electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix‐and‐inject crystallography. These results illustrate the structural basis for the ligand‐induced conformational changes associated with the molecular ‘switch’.

Sponsoring Organization:
USDOE
OSTI ID:
1399617
Journal Information:
Federation of European Biochemical Societies (FEBS) Journal, Journal Name: Federation of European Biochemical Societies (FEBS) Journal Vol. 284 Journal Issue: 20; ISSN 1742-464X
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 16 works
Citation information provided by
Web of Science

References (28)

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