The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition

Journal Article · Sat Apr 01 00:00:00 EDT 2017 · Cell Reports

DOI:https://doi.org/10.1016/j.celrep.2017.03.022· OSTI ID:1399600

Tamayo, Joel V.; Teramoto, Takamasa; Chatterjee, Seema; Hall, Traci M. Tanaka; Gavis, Elizabeth R.

The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo’s RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo’s functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Lastly, our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.

• Research Organization: Princeton Univ., NJ (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES)
• Grant/Contract Number: W-31-109-Eng-38
• OSTI ID: 1399600
• Alternate ID(s): OSTI ID: 1367528
• Journal Information: Cell Reports, Journal Name: Cell Reports Vol. 19 Journal Issue: 1; ISSN 2211-1247
• Publisher: Elsevier
• Country of Publication: Netherlands
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
glorund
nanos
Drosophila
hnRNP
hnRNP F
hnRNP H
RNA-binding protein
translational control
translational repressor
post-transcriptional regulation
development