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Title: Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles

Abstract

Controlling the traffic of molecules and ions across membranes is a critical feature in a number of biologically relevant processes and highly desirable for the development of technologies based on membrane materials. In this study, ion transport behavior of hybrid lipid/polymer membranes was studied in the absence and presence of ion transfer agents. A pH-sensitive fluorophore was used to investigate ion (H +/OH -) permeability across hybrid lipid/polymer membranes as a function of the fraction of amphiphilic block copolymer. It was observed that vesicles with intermediate lipid/polymer ratios tend to be surprisingly more permeable to ion transport than the pure lipid or pure polymer vesicles. Hybrid vesicle permeability could be further modulated with valinomycin, nigericin, or gramicidin A, which significantly expedite the dissipation of externally-imposed pH gradients by facilitating the transport of the rate-limiting co-ions (e.g. K +) ions across the membrane. For gramicidin A, ion permeability decreased with increasing polymer mole fraction, and the method of introduction of gramicidin A into the membrane played an important role. Finally, strategies to incorporate biofunctional molecules and facilitate their activity in synthetic systems are highly desirable for developing artificial organelles or other synthetic compartmentalized structures requiring control over molecular traffic across biomimeticmore » membranes.« less

Authors:
 [1];  [1];  [2];  [1];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
  2. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Nano and Microsensors Dept.
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC)
OSTI Identifier:
1399496
Report Number(s):
SAND-2017-2657J
Journal ID: ISSN 0927-7765; 651645
Grant/Contract Number:
NA0003525
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Colloids and Surfaces. B, Biointerfaces
Additional Journal Information:
Journal Volume: 159; Journal ID: ISSN 0927-7765
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; hybrid vesicles; transport; nanoreactors; polymersomes; ion channels; compartmentalization; ionophores

Citation Formats

Paxton, Walter F., McAninch, Patrick T., Achyuthan, Komandoor E., Shin, Sun Hae Ra, and Monteith, Haley L.. Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles. United States: N. p., 2017. Web. doi:10.1016/j.colsurfb.2017.07.091.
Paxton, Walter F., McAninch, Patrick T., Achyuthan, Komandoor E., Shin, Sun Hae Ra, & Monteith, Haley L.. Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles. United States. doi:10.1016/j.colsurfb.2017.07.091.
Paxton, Walter F., McAninch, Patrick T., Achyuthan, Komandoor E., Shin, Sun Hae Ra, and Monteith, Haley L.. Tue . "Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles". United States. doi:10.1016/j.colsurfb.2017.07.091.
@article{osti_1399496,
title = {Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles},
author = {Paxton, Walter F. and McAninch, Patrick T. and Achyuthan, Komandoor E. and Shin, Sun Hae Ra and Monteith, Haley L.},
abstractNote = {Controlling the traffic of molecules and ions across membranes is a critical feature in a number of biologically relevant processes and highly desirable for the development of technologies based on membrane materials. In this study, ion transport behavior of hybrid lipid/polymer membranes was studied in the absence and presence of ion transfer agents. A pH-sensitive fluorophore was used to investigate ion (H+/OH-) permeability across hybrid lipid/polymer membranes as a function of the fraction of amphiphilic block copolymer. It was observed that vesicles with intermediate lipid/polymer ratios tend to be surprisingly more permeable to ion transport than the pure lipid or pure polymer vesicles. Hybrid vesicle permeability could be further modulated with valinomycin, nigericin, or gramicidin A, which significantly expedite the dissipation of externally-imposed pH gradients by facilitating the transport of the rate-limiting co-ions (e.g. K+) ions across the membrane. For gramicidin A, ion permeability decreased with increasing polymer mole fraction, and the method of introduction of gramicidin A into the membrane played an important role. Finally, strategies to incorporate biofunctional molecules and facilitate their activity in synthetic systems are highly desirable for developing artificial organelles or other synthetic compartmentalized structures requiring control over molecular traffic across biomimetic membranes.},
doi = {10.1016/j.colsurfb.2017.07.091},
journal = {Colloids and Surfaces. B, Biointerfaces},
number = ,
volume = 159,
place = {United States},
year = {Tue Aug 01 00:00:00 EDT 2017},
month = {Tue Aug 01 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
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