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Title: Reproductive Microbiomes: Using the microbiome as a novel diagnostic tool for endometriosis

Abstract

Endometriosis is a chronic inflammatory disease which results in significant pain and long term reproductive consequences for up to 50% of infertile women. This study was focused to understand how endometriosis altered the uterine and cervical bacterial community. Urogenital swabs and uterine washes were collected from 19 pre-menopausal women undergoing laparoscopic surgery for pelvic pain, suspected endometriosis (experimental n = 10), and women undergoing laparoscopic surgery for benign ovarian/uterine conditions (control n = 9). Patients were followed for the next year and repeat cervical swabs were obtained. Bacterial community composition was assessed from these samples using Illumina next generation 16S rRNA amplicon sequencing. Bacterial communities were significantly different between sample sites, the uterus and cervix, and stage III endometriosis resulted in significant alterations in the cervical bacterial community. Both bacterial richness and phylogenetic diversity increased in association with stage III endometriosis. Surgical intervention resulted in a stabilized cervical bacterial community for a short period of time. Lastly, bacterial community profiling may provide a useful diagnostic tool for identifying endometriosis in asymptomatic, infertile women in a clinical setting.

Authors:
ORCiD logo [1];  [2];  [3];  [3];  [3];  [4];  [5]
  1. The Univ. of Illinois, Urbana, IL (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Southern Illinois Univ. School of Medicine, Springfield, IL (United States)
  3. Southern Illinois Univ. School of Medicine, Springfield, IL (United States); Michigan State College of Medicine, Grand Rapids, MI (United States)
  4. The Univ. of Illinois, Urbana, IL (United States)
  5. The Univ. of Illinois, Urbana, IL (United States); Southern Illinois Univ. of School of Medicine, Springfield, IL (United States); Spectrum Health Medical Group, Grand Rapids, MI (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1399444
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Reproductive Immunology
Additional Journal Information:
Journal Volume: 2; Journal Issue: 3:36; Journal ID: ISSN 2476-1974
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Cervix; endometriosis; Microbiome; Next generation sequencing; Uterus

Citation Formats

Cregger, Melissa, Lenz, Katherine, Leary, Elizabeth, Leach, Richard, Fazleabas, Asgerally, White, Bryan, and Braundmeier, Andrea. Reproductive Microbiomes: Using the microbiome as a novel diagnostic tool for endometriosis. United States: N. p., 2017. Web. doi:10.21767/2476-1974.100036.
Cregger, Melissa, Lenz, Katherine, Leary, Elizabeth, Leach, Richard, Fazleabas, Asgerally, White, Bryan, & Braundmeier, Andrea. Reproductive Microbiomes: Using the microbiome as a novel diagnostic tool for endometriosis. United States. doi:10.21767/2476-1974.100036.
Cregger, Melissa, Lenz, Katherine, Leary, Elizabeth, Leach, Richard, Fazleabas, Asgerally, White, Bryan, and Braundmeier, Andrea. Mon . "Reproductive Microbiomes: Using the microbiome as a novel diagnostic tool for endometriosis". United States. doi:10.21767/2476-1974.100036. https://www.osti.gov/servlets/purl/1399444.
@article{osti_1399444,
title = {Reproductive Microbiomes: Using the microbiome as a novel diagnostic tool for endometriosis},
author = {Cregger, Melissa and Lenz, Katherine and Leary, Elizabeth and Leach, Richard and Fazleabas, Asgerally and White, Bryan and Braundmeier, Andrea},
abstractNote = {Endometriosis is a chronic inflammatory disease which results in significant pain and long term reproductive consequences for up to 50% of infertile women. This study was focused to understand how endometriosis altered the uterine and cervical bacterial community. Urogenital swabs and uterine washes were collected from 19 pre-menopausal women undergoing laparoscopic surgery for pelvic pain, suspected endometriosis (experimental n = 10), and women undergoing laparoscopic surgery for benign ovarian/uterine conditions (control n = 9). Patients were followed for the next year and repeat cervical swabs were obtained. Bacterial community composition was assessed from these samples using Illumina next generation 16S rRNA amplicon sequencing. Bacterial communities were significantly different between sample sites, the uterus and cervix, and stage III endometriosis resulted in significant alterations in the cervical bacterial community. Both bacterial richness and phylogenetic diversity increased in association with stage III endometriosis. Surgical intervention resulted in a stabilized cervical bacterial community for a short period of time. Lastly, bacterial community profiling may provide a useful diagnostic tool for identifying endometriosis in asymptomatic, infertile women in a clinical setting.},
doi = {10.21767/2476-1974.100036},
journal = {Reproductive Immunology},
number = 3:36,
volume = 2,
place = {United States},
year = {Mon Sep 25 00:00:00 EDT 2017},
month = {Mon Sep 25 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
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