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Title: ‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family

Journal Article · · Systematic and Applied Microbiology
ORCiD logo [1];  [1];  [2];  [3];  [4];  [5]; ORCiD logo [6]
  1. Univ. of Tennessee and Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology
  2. Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering
  3. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
  4. Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering, School of Biology
  5. Corporate Remediation Group, Newark, DE (United States)
  6. Univ. of Tennessee and Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering
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Taxonomic assignments of anaerobic dichloromethane (DCM)-degrading bacteria remain poorly constrained but are important for understanding the microbial diversity of organisms contributing to DCM turnover in environmental systems. Here, we describe the taxonomic classification of a novel DCM degrader in consortium RM obtained from pristine Rio Mameyes sediment. Phylogenetic analysis of full-length 16S rRNA gene sequences demonstrated that the DCM degrader was most closely related to members of the genera Dehalobacter and Syntrophobotulus, but sequence similarities did not exceed 94% and 93%, respectively. Genome-aggregate average amino acid identities against Peptococcaceae members did not exceed 66%, suggesting that the DCM degrader does not affiliate with any described genus. Phylogenetic analysis of conserved single-copy functional genes supported that the DCM degrader represents a novel clade. Growth strictly depended on the presence of DCM, which was consumed at a rate of 160 ± 3 μmol L-1 d-1. The DCM degrader attained 5.25 × 107 ± 1.0 × 107 cells per μmol DCM consumed. Fluorescence in situ hybridization revealed rod-shaped cells 4 ± 0.8 μm long and 0.4 ± 0.1 μm wide. Furthermore, based on the unique phylogenetic, genomic, and physiological characteristics, we propose that the DCM degrader represents a new genus and species, ‘Candidatus Dichloromethanomonas elyunquensis’.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1399390
Journal Information:
Systematic and Applied Microbiology, Vol. 40, Issue 3; ISSN 0723-2020
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 31 works
Citation information provided by
Web of Science

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Cited By (6)

Proteogenomics Reveals Novel Reductive Dehalogenases and Methyltransferases Expressed during Anaerobic Dichloromethane Metabolism journal January 2019
Mineralization versus fermentation: evidence for two distinct anaerobic bacterial degradation pathways for dichloromethane journal January 2020
Comparative genomics reveals a novel genetic organization of the sad cluster in the sulfonamide-degrader ‘Candidatus Leucobacter sulfamidivorax’ strain GP journal November 2019
Nomenclature Abstract for Dichloromethanomonas elyunquensis Kleindienst et al. 2017. dataset January 2017
Taxonomic Abstract for the genera. dataset January 2017
Bacterial Community Dynamics in Dichloromethane-Contaminated Groundwater Undergoing Natural Attenuation journal November 2017

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
96 KNOWLEDGE MANAGEMENT AND PRESERVATION
anaerobic dichloromethane degradation
candidatus dichloromethanomonas elyunquensis
peptococcaceae
metagenome binning