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Title: Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies: Comparing lipids and metabolites in serum and DBS samples

Abstract

The use of dried blood spots (DBS) has many advantages over traditional plasma and serum samples such as smaller blood volume required, storage at room temperature, and ability for sampling in remote locations. However, understanding the robustness of different analytes in DBS samples is essential, especially in older samples collected for longitudinal studies. Here we analyzed DBS samples collected in 2000-2001 and stored at room temperature and compared them to matched serum samples stored at -80°C to determine if they could be effectively used as specific time points in a longitudinal study following metabolic disease. Four hundred small molecules were identified in both the serum and DBS samples using gas chromatograph-mass spectrometry (GC-MS), liquid chromatography-MS (LC-MS) and LC-ion mobility spectrometry-MS (LC-IMS-MS). The identified polar metabolites overlapped well between the sample types, though only one statistically significant polar metabolite in a case-control study was conserved, indicating degradation occurs in the DBS samples affecting quantitation. Differences in the lipid identifications indicated that some oxidation occurs in the DBS samples. However, thirty-six statistically significant lipids correlated in both sample types indicating that lipid quantitation was more stable across the sample types.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [3];  [4];  [4];  [1];  [1];  [1]
  1. Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland WA USA
  2. National Security Directorate, Pacific Northwest National Laboratory, Richland WA USA
  3. Department of Medicine, Bone and Mineral Unit, Oregon Health and Science University, Portland OR USA; Research Service, Portland Veterans Affairs Medical Center, Portland OR USA
  4. Department of Medicine, Bone and Mineral Unit, Oregon Health and Science University, Portland OR USA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1399180
Report Number(s):
PNNL-SA-121197
Journal ID: ISSN 0951-4198; 48199; 49531; 48680; KP1601010; 453040220; 400412000; WN9030198
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Rapid Communications in Mass Spectrometry; Journal Volume: 31; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES; Environmental Molecular Sciences Laboratory

Citation Formats

Kyle, Jennifer E., Casey, Cameron P., Stratton, Kelly G., Zink, Erika M., Kim, Young-Mo, Zheng, Xueyun, Monroe, Matthew E., Weitz, Karl K., Bloodsworth, Kent J., Orton, Daniel J., Ibrahim, Yehia M., Moore, Ronald J., Lee, Christine G., Pedersen, Catherine, Orwoll, Eric, Smith, Richard D., Burnum-Johnson, Kristin E., and Baker, Erin S. Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies: Comparing lipids and metabolites in serum and DBS samples. United States: N. p., 2017. Web. doi:10.1002/rcm.7808.
Kyle, Jennifer E., Casey, Cameron P., Stratton, Kelly G., Zink, Erika M., Kim, Young-Mo, Zheng, Xueyun, Monroe, Matthew E., Weitz, Karl K., Bloodsworth, Kent J., Orton, Daniel J., Ibrahim, Yehia M., Moore, Ronald J., Lee, Christine G., Pedersen, Catherine, Orwoll, Eric, Smith, Richard D., Burnum-Johnson, Kristin E., & Baker, Erin S. Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies: Comparing lipids and metabolites in serum and DBS samples. United States. doi:10.1002/rcm.7808.
Kyle, Jennifer E., Casey, Cameron P., Stratton, Kelly G., Zink, Erika M., Kim, Young-Mo, Zheng, Xueyun, Monroe, Matthew E., Weitz, Karl K., Bloodsworth, Kent J., Orton, Daniel J., Ibrahim, Yehia M., Moore, Ronald J., Lee, Christine G., Pedersen, Catherine, Orwoll, Eric, Smith, Richard D., Burnum-Johnson, Kristin E., and Baker, Erin S. Sun . "Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies: Comparing lipids and metabolites in serum and DBS samples". United States. doi:10.1002/rcm.7808.
@article{osti_1399180,
title = {Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies: Comparing lipids and metabolites in serum and DBS samples},
author = {Kyle, Jennifer E. and Casey, Cameron P. and Stratton, Kelly G. and Zink, Erika M. and Kim, Young-Mo and Zheng, Xueyun and Monroe, Matthew E. and Weitz, Karl K. and Bloodsworth, Kent J. and Orton, Daniel J. and Ibrahim, Yehia M. and Moore, Ronald J. and Lee, Christine G. and Pedersen, Catherine and Orwoll, Eric and Smith, Richard D. and Burnum-Johnson, Kristin E. and Baker, Erin S.},
abstractNote = {The use of dried blood spots (DBS) has many advantages over traditional plasma and serum samples such as smaller blood volume required, storage at room temperature, and ability for sampling in remote locations. However, understanding the robustness of different analytes in DBS samples is essential, especially in older samples collected for longitudinal studies. Here we analyzed DBS samples collected in 2000-2001 and stored at room temperature and compared them to matched serum samples stored at -80°C to determine if they could be effectively used as specific time points in a longitudinal study following metabolic disease. Four hundred small molecules were identified in both the serum and DBS samples using gas chromatograph-mass spectrometry (GC-MS), liquid chromatography-MS (LC-MS) and LC-ion mobility spectrometry-MS (LC-IMS-MS). The identified polar metabolites overlapped well between the sample types, though only one statistically significant polar metabolite in a case-control study was conserved, indicating degradation occurs in the DBS samples affecting quantitation. Differences in the lipid identifications indicated that some oxidation occurs in the DBS samples. However, thirty-six statistically significant lipids correlated in both sample types indicating that lipid quantitation was more stable across the sample types.},
doi = {10.1002/rcm.7808},
journal = {Rapid Communications in Mass Spectrometry},
number = 5,
volume = 31,
place = {United States},
year = {Sun Feb 05 00:00:00 EST 2017},
month = {Sun Feb 05 00:00:00 EST 2017}
}