Strengths, challenges, and opportunities for hydrothermal pretreatment in lignocellulosic biorefineries

Yang, Bin; Tao, Ling; Wyman, Charles E.

doi:10.1002/bbb.1825

Title: Strengths, challenges, and opportunities for hydrothermal pretreatment in lignocellulosic biorefineries

Journal Article · Wed Oct 11 00:00:00 EDT 2017 · Biofuels, Bioproducts & Biorefining

DOI:https://doi.org/10.1002/bbb.1825· OSTI ID:1405277

^[1]; Tao, Ling ^[2]; Wyman, Charles E. ^[3]

Washington State Univ., Richland, WA (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Pretreatment prior to or during biological conversion is required to achieve high sugar yields essential to economic production of fuels and chemicals from low cost, abundant lignocellulosic biomass. Aqueous thermochemical pretreatments achieve this performance objective from pretreatment coupled with subsequent enzymatic hydrolysis, but chemical pretreatment can also suffer from additional costs for exotic materials of construction, the need to recover or neutralize the chemicals, introduction of compounds that inhibit downstream operations, and waste disposal, as well as for the chemicals themselves. The simplicity of hydrothermal pretreatment with just hot water offers the potential to greatly improve the cost of the entire conversion process if sugar degradation during pretreatment, production of un-fermentable oligomers, and the amount of expensive enzymes needed to obtain satisfactory yields from hydrothermally pretreated solids can be reduced. Biorefinery economics would also benefit if value could be generated from lignin and other components that are currently fated to be burned for power. However, achieving these goals will no doubt require development of advanced hydrothermal pretreatment configurations. For example, passing water through a stationary bed of lignocellulosic biomass in a flowthrough configuration achieves very high yields of hemicellulose sugars, removes more than 75% of the lignin for potential valorization, and improves sugar release from the pretreated solids with lower enzyme loadings. Unfortunately, the large quantities of water needed to achieve this performance result in very dilute sugars, high energy costs for pretreatment and product recover, and large amounts of oligomers. Furthermore, improving our understanding of hydrothermal pretreatment fundamentals is needed to gain insights into R&D opportunities to improve performance, and help identify novel configurations that lower capital and operating costs and achieve higher yields.

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC36-08GO28308; EE0006112; PS02-06ER64304

OSTI ID:: 1405277

Alternate ID(s):: OSTI ID: 1399060

Report Number(s):: NREL/JA-5100-70374

Journal Information:: Biofuels, Bioproducts & Biorefining, Vol. 12, Issue 1; ISSN 1932-104X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 86 works

Citation information provided by
Web of Science

References (70)

Characterization of mineral matter particles in gasification and combustion processes Thiel, Christopher; Pohl, Martin; Grahl, Sebastian Fuel, Vol. 152 https://doi.org/10.1016/j.fuel.2015.01.025	journal	July 2015
Uncatalyzed solvolysis of whole biomass hemicellulose by hot compressed liquid water Mok, William Shu Lai; Antal, Michael Jerry Industrial & Engineering Chemistry Research, Vol. 31, Issue 4 https://doi.org/10.1021/ie00004a026	journal	April 1992
Dynamic changes of substrate reactivity and enzyme adsorption on partially hydrolyzed cellulose: Dynamics of Cellulose Reactivity and Cellulases Adsorption Shi, Jian; Wu, Dong; Zhang, Libing Biotechnology and Bioengineering, Vol. 114, Issue 3 https://doi.org/10.1002/bit.26180	journal	November 2016
Der hydrothermale Abbau von Glucose Bobleter, O.; Pape, G. Monatshefte f�r Chemie, Vol. 99, Issue 4 https://doi.org/10.1007/BF00902707	journal	January 1968
Method for Determining the Solubilities of Hydrophobic Organics in Subcritical Water Miller, David J.; Hawthorne, Steven B. Analytical Chemistry, Vol. 70, Issue 8 https://doi.org/10.1021/ac971161x	journal	April 1998
Sugar yields from dilute oxalic acid pretreatment of maple wood compared to those with other dilute acids and hot water Zhang, Taiying; Kumar, Rajeev; Wyman, Charles E. Carbohydrate Polymers, Vol. 92, Issue 1 https://doi.org/10.1016/j.carbpol.2012.09.070	journal	January 2013
Pathways for biomass-derived lignin to hydrocarbon fuels Laskar, Dhrubojyoti D.; Yang, Bin; Wang, Huamin Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 5 https://doi.org/10.1002/bbb.1422	journal	July 2013
Bioconversion of lignocellulose: inhibitors and detoxification Jönsson, Leif J.; Alriksson, Björn; Nilvebrant, Nils-Olof Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-16	journal	January 2013
Determination of the hydrothermal degradation products of D-(U-14C) glucose and D-(U-14C) fructose by TLC Bonn, G.; Bobleter, O. Journal of Radioanalytical Chemistry, Vol. 79, Issue 2 https://doi.org/10.1007/BF02518929	journal	September 1983
Pasteur's Quadrant: Basic Science and Technological Innovation Channell, David F. Technology and Culture, Vol. 40, Issue 2 https://doi.org/10.1353/tech.1999.0093	journal	January 1999
A Comparison between Hot Liquid Water and Steam Fractionation of Corn Fiber Allen, Stephen Glen; Schulman, Deborah; Lichwa, Joseph Industrial & Engineering Chemistry Research, Vol. 40, Issue 13 https://doi.org/10.1021/ie990831h	journal	June 2001
BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates Yang, Bin; Wyman, Charles E. Biotechnology and Bioengineering, Vol. 94, Issue 4 https://doi.org/10.1002/bit.20750	journal	January 2006
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
Characterization of the degree of polymerization of xylooligomers produced by flowthrough hydrolysis of pure xylan and corn stover with water Yang, Bin; Wyman, Charles E. Bioresource Technology, Vol. 99, Issue 13 https://doi.org/10.1016/j.biortech.2007.10.054	journal	September 2008
The water solubilities and heats of solution of short chain cellulosic oligosaccharides Taylor, J. B. Transactions of the Faraday Society, Vol. 53 https://doi.org/10.1039/tf9575301198	journal	January 1957
Noble-metal catalyzed hydrodeoxygenation of biomass-derived lignin to aromatic hydrocarbons Laskar, Dhrubojyoti D.; Tucker, Melvin P.; Chen, Xiaowen Green Chemistry, Vol. 16, Issue 2 https://doi.org/10.1039/c3gc42041h	journal	January 2014
Mechanism of formation of 5-(hydroxymethyl)-2-furaldehyde from d-fructose and sucrose Antal, Michael Jerry; Mok, William S. L.; Richards, Geoffrey N. Carbohydrate Research, Vol. 199, Issue 1, p. 91-109 https://doi.org/10.1016/0008-6215(90)84096-D	journal	May 1990
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels Zeng, Yining; Zhao, Shuai; Yang, Shihui Current Opinion in Biotechnology, Vol. 27 https://doi.org/10.1016/j.copbio.2013.09.008	journal	June 2014
Organic Chemical Reactions in Supercritical Water Savage, Phillip E. Chemical Reviews, Vol. 99, Issue 2 https://doi.org/10.1021/cr9700989	journal	February 1999
Influence of mineral matter on biomass pyrolysis characteristics Raveendran, K.; Ganesh, Anuradda; Khilar, Kartic C. Fuel, Vol. 74, Issue 12 https://doi.org/10.1016/0016-2361(95)80013-8	journal	December 1995
Continuous pH monitoring during pretreatment of yellow poplar wood sawdust by pressure cooking in water Weil, Joseph; Brewer, Mark; Hendrickson, Richard Applied Biochemistry and Biotechnology, Vol. 70-72, Issue 1 https://doi.org/10.1007/BF02920127	journal	March 1998
Vibrational spectral signatures of crystalline cellulose using high resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS) Zhang, Libing; Lu, Zhou; Velarde, Luis Cellulose, Vol. 22, Issue 3 https://doi.org/10.1007/s10570-015-0588-0	journal	March 2015
The fate of lignin during hydrothermal pretreatment Trajano, Heather L.; Engle, Nancy L.; Foston, Marcus Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-110	journal	January 2013
Hydrothermal degradation of polymers derived from plants Bobleter, Ortwin Progress in Polymer Science, Vol. 19, Issue 5, p. 797-841 https://doi.org/10.1016/0079-6700(94)90033-7	journal	January 1994
Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification Palmqvist, Eva; Hahn-Hägerdal, Bärbel Bioresource Technology, Vol. 74, Issue 1, p. 17-24 https://doi.org/10.1016/S0960-8524(99)00160-1	journal	August 2000
Retention mechanisms in subcritical water reversed-phase chromatography Allmon, Steven D.; Dorsey, John G. Journal of Chromatography A, Vol. 1216, Issue 26 https://doi.org/10.1016/j.chroma.2009.04.068	journal	June 2009
Analytical methodology for biomass pretreatment. Part 2: Characterization of the filtrates and cumulative product distribution as a function of treatment severity Bouchard, Jean; Nguyen, Thuc S.; Chornet, Esteban Bioresource Technology, Vol. 36, Issue 2 https://doi.org/10.1016/0960-8524(91)90169-K	journal	January 1991
Ion product of water substance, 0–1000 °C, 1–10,000 bars New International Formulation and its background Marshall, William L.; Franck, E. U. Journal of Physical and Chemical Reference Data, Vol. 10, Issue 2 https://doi.org/10.1063/1.555643	journal	April 1981
Physiochemical Characterization of Lignocellulosic Biomass Dissolution by Flowthrough Pretreatment Yan, Lishi; Pu, Yunqiao; Bowden, Mark ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 1 https://doi.org/10.1021/acssuschemeng.5b01021	journal	December 2015
Pasteur's Quadrant: Basic science and technological innovation Brandl, John E. Journal of Policy Analysis and Management, Vol. 17, Issue 4 https://doi.org/10.1002/(sici)1520-6688(199823)17:4<734::aid-pam10>3.0.co;2-g	journal	October 1998
Enhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment Yan, Lishi; Zhang, Libing; Yang, Bin Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-76	journal	January 2014
Revealing the Molecular Structural Transformation of Hardwood and Softwood in Dilute Acid Flowthrough Pretreatment Zhang, Libing; Pu, Yunqiao; Cort, John R. ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 12 https://doi.org/10.1021/acssuschemeng.6b01491	journal	October 2016
Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose Yang, Bin; Wyman, Charles E. Biotechnology and Bioengineering, Vol. 86, Issue 1 https://doi.org/10.1002/bit.20043	journal	January 2004
Likely features and costs of mature biomass ethanol technology Lynd, Lee R.; Elamder, Richard T.; Wyman, Charles E. Applied Biochemistry and Biotechnology, Vol. 57-58, Issue 1 https://doi.org/10.1007/BF02941755	journal	March 1996
Lignin Valorization: Improving Lignin Processing in the Biorefinery Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J. Science, Vol. 344, Issue 6185, p. 1246843-1246843 https://doi.org/10.1126/science.1246843	journal	May 2014
Solubilities of Oligomer Mixtures Produced by the Hydrolysis of Xylans and Corn Stover in Water at 180 °C Gray, Matthew C.; Converse, Alvin O.; Wyman, Charles E. Industrial & Engineering Chemistry Research, Vol. 46, Issue 8 https://doi.org/10.1021/ie060325+	journal	March 2007
Cellulose pretreatment in subcritical water: Effect of temperature on molecular structure and enzymatic reactivity Kumar, Sandeep; Gupta, Rajesh; Lee, Y. Y. Bioresource Technology, Vol. 101, Issue 4 https://doi.org/10.1016/j.biortech.2009.09.035	journal	February 2010
Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass Kim, Youngmi; Ximenes, Eduardo; Mosier, Nathan S. Enzyme and Microbial Technology, Vol. 48, Issue 4-5 https://doi.org/10.1016/j.enzmictec.2011.01.007	journal	April 2011
Characterization of lignin derived from water-only and dilute acid flowthrough pretreatment of poplar wood at elevated temperatures Zhang, Libing; Yan, Lishi; Wang, Zheming Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0377-x	journal	December 2015
Hydrothermolysis ? a new process for the utilization of Biomass Bonn, G.; Concin, R.; Bobleter, O. Wood Science and Technology, Vol. 17, Issue 3 https://doi.org/10.1007/BF00372318	journal	January 1983
Fluids at high pressures and temperatures Ulrich Franck, E. The Journal of Chemical Thermodynamics, Vol. 19, Issue 3 https://doi.org/10.1016/0021-9614(87)90130-3	journal	March 1987
What is (and is not) vital to advancing cellulosic ethanol Wyman, Charles E. Trends in Biotechnology, Vol. 25, Issue 4 https://doi.org/10.1016/j.tibtech.2007.02.009	journal	April 2007
Dissolution and Hydrolysis of Cellulose in Subcritical and Supercritical Water Sasaki, Mitsuru; Fang, Zhen; Fukushima, Yoshiko Industrial & Engineering Chemistry Research, Vol. 39, Issue 8 https://doi.org/10.1021/ie990690j	journal	August 2000
The Effect of Flow Rate of Compressed Hot Water on Xylan, Lignin, and Total Mass Removal from Corn Stover Liu, Chaogang; Wyman, Charles E. Industrial & Engineering Chemistry Research, Vol. 42, Issue 21, p. 5409-5416 https://doi.org/10.1021/ie030458k	journal	September 2003
Two-step hydrolysis of Japanese cedar as treated by semi-flow hot-compressed water Phaiboonsilpa, Natthanon; Yamauchi, Kazuchika; Lu, Xin Journal of Wood Science, Vol. 56, Issue 4 https://doi.org/10.1007/s10086-009-1099-0	journal	March 2010
Enzymatic hydrolysis of cellulosic biomass Yang, Bin; Dai, Ziyu; Ding, Shi-You Biofuels, Vol. 2, Issue 4 https://doi.org/10.4155/bfs.11.116	journal	July 2011
Features of promising technologies for pretreatment of lignocellulosic biomass Mosier, Nathan; Wyman, Charles; Dale, Bruce Bioresource Technology, Vol. 96, Issue 6, p. 673-686 https://doi.org/10.1016/j.biortech.2004.06.025	journal	April 2005
Fractionation of Sugar Cane with Hot, Compressed, Liquid Water Allen, Stephen Glen; Kam, Lance Cameron; Zemann, Andreas Joseph Industrial & Engineering Chemistry Research, Vol. 35, Issue 8 https://doi.org/10.1021/ie950594s	journal	January 1996
Characterization of Molecular Weight Distribution of Oligomers from Autocatalyzed Batch Hydrolysis of Xylan Li, Xia; Converse, Alvin O.; Wyman, Charles E. Applied Biochemistry and Biotechnology, Vol. 107, Issue 1-3 https://doi.org/10.1385/ABAB:107:1-3:515	journal	January 2003
Dehydration of d-glucose in high temperature water at pressures up to 80MPa Aida, Taku Michael; Sato, Yukiko; Watanabe, Masaru The Journal of Supercritical Fluids, Vol. 40, Issue 3 https://doi.org/10.1016/j.supflu.2006.07.027	journal	April 2007
Fluids at high pressures and temperatures Franck, E. U. Pure and Applied Chemistry, Vol. 59, Issue 1 https://doi.org/10.1351/pac198759010025	journal	January 1987
The enhancement of xylose monomer and xylotriose degradation by inorganic salts in aqueous solutions at 180 °C Liu, Chaogang; Wyman, Charles E. Carbohydrate Research, Vol. 341, Issue 15 https://doi.org/10.1016/j.carres.2006.07.017	journal	November 2006
Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges Wyman, Charles E. Annual Review of Energy and the Environment, Vol. 24, Issue 1, p. 189-226 https://doi.org/10.1146/annurev.energy.24.1.189	journal	November 1999
Process and technoeconomic analysis of leading pretreatment technologies for lignocellulosic ethanol production using switchgrass Tao, Ling; Aden, Andy; Elander, Richard T. Bioresource Technology, Vol. 102, Issue 24, p. 11105-11114 https://doi.org/10.1016/j.biortech.2011.07.051	journal	December 2011
Fermentable hexose production from corn stalks and wheat straw with combined supercritical and subcritical hydrothermal technology Zhao, Yan; Lu, Wen-Jing; Wang, Hong-Tao Bioresource Technology, Vol. 100, Issue 23 https://doi.org/10.1016/j.biortech.2009.06.079	journal	December 2009
Biocommodity Engineering Lynd, L. R.; Wyman, C. E.; Gerngross, T. U. Biotechnology Progress, Vol. 15, Issue 5 https://doi.org/10.1021/bp990109e	journal	October 1999
Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology: Selected Key Events Along the Way Wyman, Charles E. Applied Biochemistry and Biotechnology, Vol. 91-93, Issue 1-9 https://doi.org/10.1385/ABAB:91-93:1-9:5	journal	January 2001
Dilute Acid and Hydrothermal Pretreatment of Cellulosic Biomass Tanjore, Deepti; Shi, Jian; Wyman, Charles E. Chemical and Biochemical Catalysis for Next Generation Biofuels https://doi.org/10.1039/9781849732857-00064	book	August 2011
Bioconversion of lignin model compounds with oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Applied Microbiology and Biotechnology, Vol. 93, Issue 2 https://doi.org/10.1007/s00253-011-3743-z	journal	December 2011
Two-step hydrolysis of Japanese beech as treated by semi-flow hot-compressed water Lu, Xin; Yamauchi, Kazuchika; Phaiboonsilpa, Natthanon Journal of Wood Science, Vol. 55, Issue 5 https://doi.org/10.1007/s10086-009-1040-6	journal	October 2009
Efficacy of a Hot Washing Process for Pretreated Yellow Poplar to Enhance Bioethanol Production Nagle, N. J.; Elander, R. T.; Newman, M. M. Biotechnology Progress, Vol. 18, Issue 4 https://doi.org/10.1021/bp0155078	journal	August 2002
Partial flow of compressed-hot water through corn stover to enhance hemicellulose sugar recovery and enzymatic digestibility of cellulose Liu, Chaogang; Wyman, Charles E. Bioresource Technology, Vol. 96, Issue 18, p. 1978-1985 https://doi.org/10.1016/j.biortech.2005.01.012	journal	December 2005
Pretreatment: the key to unlocking low-cost cellulosic ethanol Yang, Bin; Wyman, Charles E. Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1 https://doi.org/10.1002/bbb.49	journal	January 2008
Kinetic modeling of xylan hydrolysis in co- and countercurrent liquid hot water flow-through pretreatments Shao, Xiongjun; Lynd, Lee Bioresource Technology, Vol. 130 https://doi.org/10.1016/j.biortech.2012.11.109	journal	February 2013
Process and economic analysis of pretreatment technologies Eggeman, Tim; Elander, Richard T. Bioresource Technology, Vol. 96, Issue 18, p. 2019-2025 https://doi.org/10.1016/j.biortech.2005.01.017	journal	December 2005
Pretreatment of yellow poplar sawdust by pressure cooking in water Weil, Joe; Sarikaya, Ayda; Rau, Shiang-Lan Applied Biochemistry and Biotechnology, Vol. 68, Issue 1-2 https://doi.org/10.1007/BF02785978	journal	October 1997
Impact of Fluid Velocity on Hot Water Only Pretreatment of Corn Stover in a Flowthrough Reactor Liu, Chaogang; Wyman, Charles E. Applied Biochemistry and Biotechnology, Vol. 115, Issue 1-3 https://doi.org/10.1385/ABAB:115:1-3:0977	journal	January 2004
Supercritical Water Treatment of Biomass for Energy and Material Recovery Matsumura, Yukihiko; Sasaki, Mitsuru; Okuda, Kazuhide Combustion Science and Technology, Vol. 178, Issue 1-3 https://doi.org/10.1080/00102200500290815	journal	January 2006
Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover Humbird, D.; Davis, R.; Tao, L. https://doi.org/10.2172/1013269	report	March 2011

Cited By (9)

Effects of Hydrothermal Pretreatment on the Structural Characteristics of Organosolv Lignin from Triarrhena lutarioriparia Chen, Tianying; Li, Zhiwen; Zhang, Xueming Polymers, Vol. 10, Issue 10 https://doi.org/10.3390/polym10101157	journal	October 2018
Carbohydrate stabilization extends the kinetic limits of chemical polysaccharide depolymerization Questell-Santiago, Ydna M.; Zambrano-Varela, Raquel; Talebi Amiri, Masoud Nature Chemistry, Vol. 10, Issue 12 https://doi.org/10.1038/s41557-018-0134-4	journal	September 2018
Enhancing Lignocellulosic Biomass Hydrolysis by Hydrothermal Pretreatment, Extraction of Surface Lignin, Wet Milling and Production of Cellulolytic Enzymes Nitsos, Christos K.; Lazaridis, Polykarpos A.; Mach‐Aigner, Astrid ChemSusChem, Vol. 12, Issue 6 https://doi.org/10.1002/cssc.201802597	journal	February 2019
Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger de Oliveira Gorgulho Silva, Caio; de Castro Moreira dos Santos Júnior, Agenor; Santana, Renata Henrique Journal of Industrial Microbiology & Biotechnology, Vol. 46, Issue 11 https://doi.org/10.1007/s10295-019-02207-0	journal	June 2019
A roadmap for renewable C2–C3 glycols production: a process engineering approach Marchesan, Andressa Neves; Oncken, Marina Pinho; Maciel Filho, Rubens Green Chemistry, Vol. 21, Issue 19 https://doi.org/10.1039/c9gc02949d	journal	January 2019
Techno‐economic analysis of jet‐fuel production from biorefinery waste lignin Shen, Rongchun; Tao, Ling; Yang, Bin Biofuels, Bioproducts and Biorefining, Vol. 13, Issue 3 https://doi.org/10.1002/bbb.1952	journal	December 2018
Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains Li, Xiaolu; He, Yucai; Zhang, Libing Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1395-x	journal	March 2019
Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes Djajadi, Demi T.; Jensen, Mads M.; Oliveira, Marlene Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1085-0	journal	April 2018
Enzyme biotechnology in degradation and modification of plant cell wall polymers Marjamaa, Kaisa; Kruus, Kristiina Physiologia Plantarum, Vol. 164, Issue 1 https://doi.org/10.1111/ppl.12800	journal	August 2018

Similar Records

Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance

Journal Article · Thu Nov 10 00:00:00 EST 2016 · Biotechnology for Biofuels · OSTI ID:1405277

Bhagia, Samarthya; Li, Hongjia; Gao, Xiadi; +2 more

IMPROVED BIOREFINERY FOR THE PRODUCTION OF ETHANOL, CHEMICALS, ANIMAL FEED AND BIOMATERIALS FROM SUGAR CANE

Title: Strengths, challenges, and opportunities for hydrothermal pretreatment in lignocellulosic biorefineries

Citation Formats

References (70)

Cited By (9)

Similar Records

Related Subjects