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Title: Site-specific incorporation of redox active amino acids into proteins

Patent ·
OSTI ID:1398985
; ;

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

Research Organization:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute
Patent Number(s):
9,783,798
Application Number:
13/344,436
OSTI ID:
1398985
Resource Relation:
Patent File Date: 2012 Jan 05
Country of Publication:
United States
Language:
English

References (19)

In vivo incorporation of unnatural amino acids patent May 2006
In vivo incorporation of unnatural amino acids patent-application May 2003
Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application June 2003
Site-Specific Incorporation of a Redox-Active Amino Acid into Proteins journal December 2003
An Expanded Eukaryotic Genetic Code journal August 2003
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change journal May 2003
Programming peptidomimetic syntheses by translating genetic codes designed de novo journal May 2003
Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation journal November 2002
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system journal July 2002
Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria journal January 2001
A general approach for the generation of orthogonal tRNAs journal September 2001
A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins journal May 2000
Expanding the Genetic Code of Escherichia coli journal April 2001
The Selective Incorporation of Alkenes into Proteins in Escherichia coli journal August 2002
A New Strategy for the Site-Specific Modification of Proteins in Vivo journal June 2003
Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli journal February 1998
A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code journal September 2000
The reciprocal exclusion by L-dopa (L-3,4-dihydroxyphenylalanine) and L-tyrosine of their incorporation as single units into a soluble rat brain protein journal July 1975
Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli journal March 2002

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