Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2

Lo, Yu-Hua; Romes, Erin M.; Pillon, Monica C.; Sobhany, Mack; Stanley, Robin E.

doi:10.1016/j.str.2017.03.008

Title: Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2

Journal Article · Thu Apr 13 00:00:00 EDT 2017 · Structure

DOI:https://doi.org/10.1016/j.str.2017.03.008· OSTI ID:1435653

Lo, Yu-Hua; Romes, Erin M.; Pillon, Monica C.; Sobhany, Mack; Stanley, Robin E.

Ribosome assembly is a complex process that requires hundreds of essential assembly factors, including Rix7 (NVL2 in mammals) and Nsa1 (WDR74 in mammals). Rix7 is a type II double ring, AAA-ATPase, which is closely related to the well-known Cdc48/p97. Previous studies in Saccharomyces cerevisiae suggest that Rix7 mediates the release of Nsa1 from nucleolar pre-60S particles; however, the underlying mechanisms of this release are unknown. Through multiple structural analyses we show that S. cerevisiae Nsa1 is composed of an N-terminal seven-bladed WD40 domain followed by a lysine-rich C terminus that extends away from the WD40 domain and is required for nucleolar localization. Additional co-immunoprecipitation assays with the mammalian homologs identified a well-conserved interface within WDR74 that is important for its association with NVL2. We further show that WDR74 associates with the D1 AAA domain of NVL2, which represents a novel mode of binding of a substrate with a type II AAA-ATPase.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institute of Environmental Health Sciences (NIEHS); National Institutes of Health (NIHI)

Grant/Contract Number:: W-31-109-Eng-38; AC02-05CH11231; ZIA ES103247; S10OD018483

OSTI ID:: 1435653

Alternate ID(s):: OSTI ID: 1368256; OSTI ID: 1397654

Journal Information:: Structure, Journal Name: Structure Vol. 25 Journal Issue: 5; ISSN 0969-2126

Publisher:: ElsevierCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 16 works

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Title: Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2

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