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Title: Investigation on hemolytic effect of poly(lactic co-glycolic) acid nanoparticles synthesized using continuous flow and batch processes

Abstract

Abstract With the increasing interest in polymeric nanoparticles for biomedical applications, there is a need for continuous flow methodologies that allow for the precise control of nanoparticle synthesis. Poly(lactide-co-glycolic) acid (PLGA) nanoparticles with diameters of 220–250 nm were synthesized using a lab-on-a-chip, exploiting the precise flow control offered by a millifluidic platform. The association and the effect of PLGA nanoparticles on red blood cells (RBCs) were compared for fluorescent PLGA nanoparticles made by this novel continuous flow process using a millifluidic chip and smaller PLGA nanoparticles made by a batch method. Results indicated that all PLGA nanoparticles studied, independent of the synthesis method and size, adhered to the surface of RBCs but had no significant hemolytic effect at concentrations lower than 10 mg/ml.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Atomic-Level Catalyst Design (CALCD)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1397170
DOE Contract Number:  
SC0001058
Resource Type:
Journal Article
Journal Name:
Nanotechnology Reviews
Additional Journal Information:
Journal Volume: 6; Journal Issue: 2; Related Information: CALCD partners with Louisiana State University (lead); Clemson University; University of Florida; Georgia Institute of Technology; Grambling State University; Oak Ridge National Laboratory; Ohio State University; Pennsylvania State; Texas A&M University; Vienna University of Technology, Austria; University of Utrecht, Netherlands; Journal ID: ISSN 2191-9089
Country of Publication:
United States
Language:
English

Citation Formats

Libi, Sumit, Calenic, Bogdan, Astete, Carlos E., Kumar, Challa, and Sabliov, Cristina M. Investigation on hemolytic effect of poly(lactic co-glycolic) acid nanoparticles synthesized using continuous flow and batch processes. United States: N. p., 2017. Web. doi:10.1515/ntrev-2016-0045.
Libi, Sumit, Calenic, Bogdan, Astete, Carlos E., Kumar, Challa, & Sabliov, Cristina M. Investigation on hemolytic effect of poly(lactic co-glycolic) acid nanoparticles synthesized using continuous flow and batch processes. United States. doi:10.1515/ntrev-2016-0045.
Libi, Sumit, Calenic, Bogdan, Astete, Carlos E., Kumar, Challa, and Sabliov, Cristina M. Sun . "Investigation on hemolytic effect of poly(lactic co-glycolic) acid nanoparticles synthesized using continuous flow and batch processes". United States. doi:10.1515/ntrev-2016-0045.
@article{osti_1397170,
title = {Investigation on hemolytic effect of poly(lactic co-glycolic) acid nanoparticles synthesized using continuous flow and batch processes},
author = {Libi, Sumit and Calenic, Bogdan and Astete, Carlos E. and Kumar, Challa and Sabliov, Cristina M.},
abstractNote = {Abstract With the increasing interest in polymeric nanoparticles for biomedical applications, there is a need for continuous flow methodologies that allow for the precise control of nanoparticle synthesis. Poly(lactide-co-glycolic) acid (PLGA) nanoparticles with diameters of 220–250 nm were synthesized using a lab-on-a-chip, exploiting the precise flow control offered by a millifluidic platform. The association and the effect of PLGA nanoparticles on red blood cells (RBCs) were compared for fluorescent PLGA nanoparticles made by this novel continuous flow process using a millifluidic chip and smaller PLGA nanoparticles made by a batch method. Results indicated that all PLGA nanoparticles studied, independent of the synthesis method and size, adhered to the surface of RBCs but had no significant hemolytic effect at concentrations lower than 10 mg/ml.},
doi = {10.1515/ntrev-2016-0045},
journal = {Nanotechnology Reviews},
issn = {2191-9089},
number = 2,
volume = 6,
place = {United States},
year = {2017},
month = {1}
}