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Title: Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community

Abstract

Hypersaline microbial mats have been shown to produce significant quantities of H 2 under dark, anoxic conditions via cyanobacterial fermentation. This flux of a widely accessible microbial substrate has potential to significantly influence the ecology of the mat, and any consumption will affect the net efflux of H 2 that might otherwise be captured as a resource. Here, we focus on H 2 consumption in a microbial mat from Elkhorn Slough, California, USA, for which H 2 production has been previously characterized. Active biologic H 2 consumption in this mat is indicated by a significant time-dependent decrease in added H 2 compared with a killed control. Inhibition of sulfate reduction, as indicated by a decrease in hydrogen sulfide production relative to controls, resulted in a significant increase in H 2 efflux, suggesting that sulfate-reducing bacteria (SRB) are important hydrogenotrophs. Low methane efflux under these same conditions indicated that methanogens are likely not important hydrogenotrophs. Analyses of genes and transcripts that encode for rRNA or dissimilatory sulfite reductase, using both PCR-dependent and PCR-independent metatranscriptomic sequencing methods, demonstrated that Desulfobacterales are the dominant, active SRB in the upper, H 2-producing layer of the mat (0–2 mm). This hypothesis was further supported bymore » the identification of transcripts encoding hydrogenases derived from Desulfobacterales capable of H 2 oxidation. Analysis of molecular data provided no evidence for the activity of hydrogenotrophic methanogens. Lastly, the combined biogeochemical and molecular data strongly indicate that SRB belonging to the Desulfobacterales are the quantitatively important hydrogenotrophs in the Elkhorn Slough mat.« less

Authors:
 [1];  [1];  [2];  [3];  [4];  [5];  [2];  [5];  [4];  [5]
  1. Stanford Univ., CA (United States). Dept. of Chemical, Civil and Environmental Engineering; NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States). Exobiology Branch
  2. Stanford Univ., CA (United States). Dept. of Chemical, Civil and Environmental Engineering
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Sciences Division
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Chemical Sciences Division
  5. NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States). Exobiology Branch
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1396205
Report Number(s):
LLNL-JRNL-605212
Journal ID: ISSN 1472-4677
Grant/Contract Number:  
AC52-07NA27344; AC02-05CH11231; SCW1039
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Geobiology
Additional Journal Information:
Journal Volume: 12; Journal Issue: 3; Journal ID: ISSN 1472-4677
Publisher:
Wiliey
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY

Citation Formats

Burow, L. C., Woebken, D., Marshall, I. P. G., Singer, S. W., Pett-Ridge, J., Prufert-Bebout, L., Spormann, A. M., Bebout, B. M., Weber, P. K., and Hoehler, T. M. Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community. United States: N. p., 2014. Web. doi:10.1111/gbi.12080.
Burow, L. C., Woebken, D., Marshall, I. P. G., Singer, S. W., Pett-Ridge, J., Prufert-Bebout, L., Spormann, A. M., Bebout, B. M., Weber, P. K., & Hoehler, T. M. Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community. United States. doi:10.1111/gbi.12080.
Burow, L. C., Woebken, D., Marshall, I. P. G., Singer, S. W., Pett-Ridge, J., Prufert-Bebout, L., Spormann, A. M., Bebout, B. M., Weber, P. K., and Hoehler, T. M. Tue . "Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community". United States. doi:10.1111/gbi.12080. https://www.osti.gov/servlets/purl/1396205.
@article{osti_1396205,
title = {Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community},
author = {Burow, L. C. and Woebken, D. and Marshall, I. P. G. and Singer, S. W. and Pett-Ridge, J. and Prufert-Bebout, L. and Spormann, A. M. and Bebout, B. M. and Weber, P. K. and Hoehler, T. M.},
abstractNote = {Hypersaline microbial mats have been shown to produce significant quantities of H2 under dark, anoxic conditions via cyanobacterial fermentation. This flux of a widely accessible microbial substrate has potential to significantly influence the ecology of the mat, and any consumption will affect the net efflux of H2 that might otherwise be captured as a resource. Here, we focus on H2 consumption in a microbial mat from Elkhorn Slough, California, USA, for which H2 production has been previously characterized. Active biologic H2 consumption in this mat is indicated by a significant time-dependent decrease in added H2 compared with a killed control. Inhibition of sulfate reduction, as indicated by a decrease in hydrogen sulfide production relative to controls, resulted in a significant increase in H2 efflux, suggesting that sulfate-reducing bacteria (SRB) are important hydrogenotrophs. Low methane efflux under these same conditions indicated that methanogens are likely not important hydrogenotrophs. Analyses of genes and transcripts that encode for rRNA or dissimilatory sulfite reductase, using both PCR-dependent and PCR-independent metatranscriptomic sequencing methods, demonstrated that Desulfobacterales are the dominant, active SRB in the upper, H2-producing layer of the mat (0–2 mm). This hypothesis was further supported by the identification of transcripts encoding hydrogenases derived from Desulfobacterales capable of H2 oxidation. Analysis of molecular data provided no evidence for the activity of hydrogenotrophic methanogens. Lastly, the combined biogeochemical and molecular data strongly indicate that SRB belonging to the Desulfobacterales are the quantitatively important hydrogenotrophs in the Elkhorn Slough mat.},
doi = {10.1111/gbi.12080},
journal = {Geobiology},
number = 3,
volume = 12,
place = {United States},
year = {Tue Apr 15 00:00:00 EDT 2014},
month = {Tue Apr 15 00:00:00 EDT 2014}
}

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Works referenced in this record:

Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969


Basic local alignment search tool
journal, October 1990

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