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Title: Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus

Abstract

Use of oleaginous microorganisms as “micro-factories” for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400–500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9–11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~more » 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [2]; ORCiD logo [3]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical & Biomolecular Engineering
  2. Texas A & M Univ., College Station, TX (United States). Dept. of Plant Pathology & Microbiology
  3. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical & Biomolecular Engineering. Inst. of Agriculture. Center for Renewable Carbon. Dept. of Forestry, Wildlife, and Fisheries; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences. Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Texas A & M Univ., College Station, TX (United States); Univ. of Tennessee, Knoxville, TN (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1395608
Alternate Identifier(s):
OSTI ID: 1471890
Grant/Contract Number:  
AC05-00OR22725; EE0006112
Resource Type:
Journal Article: Published Article
Journal Name:
AMB Express
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2191-0855
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; dilute-acid pretreatment; simultaneous saccharification and fermentation; Rhodococcus opacus; biofuel; lipids

Citation Formats

Le, Rosemary K., Das, Parthapratim, Mahan, Kristina M., Anderson, Seth A., Wells, Tyrone, Yuan, Joshua S., and Ragauskas, Arthur J. Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus. United States: N. p., 2017. Web. doi:10.1186/s13568-017-0484-0.
Le, Rosemary K., Das, Parthapratim, Mahan, Kristina M., Anderson, Seth A., Wells, Tyrone, Yuan, Joshua S., & Ragauskas, Arthur J. Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus. United States. doi:10.1186/s13568-017-0484-0.
Le, Rosemary K., Das, Parthapratim, Mahan, Kristina M., Anderson, Seth A., Wells, Tyrone, Yuan, Joshua S., and Ragauskas, Arthur J. Fri . "Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus". United States. doi:10.1186/s13568-017-0484-0.
@article{osti_1395608,
title = {Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus},
author = {Le, Rosemary K. and Das, Parthapratim and Mahan, Kristina M. and Anderson, Seth A. and Wells, Tyrone and Yuan, Joshua S. and Ragauskas, Arthur J.},
abstractNote = {Use of oleaginous microorganisms as “micro-factories” for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400–500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9–11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~ 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.},
doi = {10.1186/s13568-017-0484-0},
journal = {AMB Express},
number = ,
volume = 7,
place = {United States},
year = {Fri Sep 29 00:00:00 EDT 2017},
month = {Fri Sep 29 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1186/s13568-017-0484-0

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Works referenced in this record:

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