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Title: Shared Features of Cryptic Plasmids from Environmental and Pathogenic Francisella Species

Abstract

The Francisella genus includes several recognized species, additional potential species, and other representatives that inhabit a range of incredibly diverse ecological niches, but are not closely related to the named species. Francisella species have been obtained from a wide variety of clinical and environmental sources; documented species include highly virulent human and animal pathogens, fish pathogens, opportunistic human pathogens, tick endosymbionts, and free-living isolates inhabiting brackish water. While more than 120 Francisella genomes have been sequenced to date, only a few contain plasmids, and most of these appear to be cryptic, with unknown benefit to the host cell. We have identified several putative cryptic plasmids in the sequenced genomes of three Francisella novicida and F. novicida-like strains (TX07-6608, AZ06-7470, DPG_3A-IS) and two new Francisella species (F. frigiditurris CA97-1460 and F. opportunistica MA06-7296). These plasmids were compared to each other and to previously identified plasmids from other Francisella species. Some of the plasmids encoded functions potentially involved in replication, conjugal transfer and partitioning, environmental survival (transcriptional regulation, signaling, metabolism), and hypothetical proteins with no assignable functions. In conclusion, genomic and phylogenetic comparisons of these new plasmids to the other known Francisella plasmids revealed some similarities that add to our understanding ofmore » the evolutionary relationships among the diverse Francisella species.« less

Authors:
ORCiD logo [1];  [2];  [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. US Food and Drug Administration, Silver Spring, MD (United States). Office of Laboratory Science and Safety
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE; US Department of Homeland Security (DHS)
OSTI Identifier:
1394974
Report Number(s):
LA-UR-17-23160
Journal ID: ISSN 1932-6203
Grant/Contract Number:
AC52-06NA25396
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 8; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Biological Science

Citation Formats

Challacombe, Jean Faust, Pillai, Segaran, and Kuske, Cheryl R. Shared Features of Cryptic Plasmids from Environmental and Pathogenic Francisella Species. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0183554.
Challacombe, Jean Faust, Pillai, Segaran, & Kuske, Cheryl R. Shared Features of Cryptic Plasmids from Environmental and Pathogenic Francisella Species. United States. doi:10.1371/journal.pone.0183554.
Challacombe, Jean Faust, Pillai, Segaran, and Kuske, Cheryl R. Thu . "Shared Features of Cryptic Plasmids from Environmental and Pathogenic Francisella Species". United States. doi:10.1371/journal.pone.0183554. https://www.osti.gov/servlets/purl/1394974.
@article{osti_1394974,
title = {Shared Features of Cryptic Plasmids from Environmental and Pathogenic Francisella Species},
author = {Challacombe, Jean Faust and Pillai, Segaran and Kuske, Cheryl R.},
abstractNote = {The Francisella genus includes several recognized species, additional potential species, and other representatives that inhabit a range of incredibly diverse ecological niches, but are not closely related to the named species. Francisella species have been obtained from a wide variety of clinical and environmental sources; documented species include highly virulent human and animal pathogens, fish pathogens, opportunistic human pathogens, tick endosymbionts, and free-living isolates inhabiting brackish water. While more than 120 Francisella genomes have been sequenced to date, only a few contain plasmids, and most of these appear to be cryptic, with unknown benefit to the host cell. We have identified several putative cryptic plasmids in the sequenced genomes of three Francisella novicida and F. novicida-like strains (TX07-6608, AZ06-7470, DPG_3A-IS) and two new Francisella species (F. frigiditurris CA97-1460 and F. opportunistica MA06-7296). These plasmids were compared to each other and to previously identified plasmids from other Francisella species. Some of the plasmids encoded functions potentially involved in replication, conjugal transfer and partitioning, environmental survival (transcriptional regulation, signaling, metabolism), and hypothetical proteins with no assignable functions. In conclusion, genomic and phylogenetic comparisons of these new plasmids to the other known Francisella plasmids revealed some similarities that add to our understanding of the evolutionary relationships among the diverse Francisella species.},
doi = {10.1371/journal.pone.0183554},
journal = {PLoS ONE},
number = 8,
volume = 12,
place = {United States},
year = {Thu Aug 24 00:00:00 EDT 2017},
month = {Thu Aug 24 00:00:00 EDT 2017}
}

Journal Article:
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