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Title: Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements

Authors:
;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1394715
Grant/Contract Number:
FG02-02ER63445
Resource Type:
Journal Article: Published Article
Journal Name:
Cell Systems
Additional Journal Information:
Journal Volume: 3; Journal Issue: 2; Related Information: CHORUS Timestamp: 2017-09-25 16:48:07; Journal ID: ISSN 2405-4712
Publisher:
Elsevier
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Ma, Natalie Jing, and Isaacs, Farren J. Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements. Country unknown/Code not available: N. p., 2016. Web. doi:10.1016/j.cels.2016.06.009.
Ma, Natalie Jing, & Isaacs, Farren J. Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements. Country unknown/Code not available. doi:10.1016/j.cels.2016.06.009.
Ma, Natalie Jing, and Isaacs, Farren J. Mon . "Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements". Country unknown/Code not available. doi:10.1016/j.cels.2016.06.009.
@article{osti_1394715,
title = {Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements},
author = {Ma, Natalie Jing and Isaacs, Farren J.},
abstractNote = {},
doi = {10.1016/j.cels.2016.06.009},
journal = {Cell Systems},
number = 2,
volume = 3,
place = {Country unknown/Code not available},
year = {Mon Aug 01 00:00:00 EDT 2016},
month = {Mon Aug 01 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.cels.2016.06.009

Citation Metrics:
Cited by: 4works
Citation information provided by
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  • Studies were made on the genetic consequences of methotrexate-directed thymidylate stress, focusing attention on a human thymidylate synthase gene that was introduced as a heterologous genetic marker into mouse thymidylate synthase-negative mutant cells. Thymidylate stress induced thymidylate synthase-negative segregants with concomitant loss of human thymidylate synthase activity with frequencies 1 to 2 orders of magnitude higher than the uninduced spontaneous level in some but not all transformant lines. Induction of the segregants was suppressed almost completely by cycloheximide and partially by caffeine. Thymidylate stress did not, however, induce mutations, as determined by measuring resistance to ouabain or 6-thioguanine. Thymidylate synthase-negativemore » segregants were also induced by other means such as bromodeoxyuridine treatment and X-ray irradiation. In each of the synthase-negative segregants induced by thymidylate stress, a DNA segment including almost the whole coding region of the transferred human thymidylate synthase gene was deleted in a very specific manner, as shown by Southern blot analysis with a human Alu sequence and a human thymidylate synthase cDNA as probes. In the segregants that emerged spontaneously at low frequency, the entire transferred genetic marker was lost. In the segregants induced by X-ray irradiation, structural alterations of the genetic marker were random. These results show that thymidylate stress is a physiological factor that provokes the instability of this exogenously incorporated DNA in some specific manner and produces nonrandom genetic recombination in mammalian cells.« less
  • The relationship between 2-D and 3-D wave propagation in horizontally layered media was first investigated by Dampney (1971). In the last few years the usefulness and feasibility of transforming point-source responses with 3-D geometric spreading to equivalent line-source responses with 2-D geometric spreading have been thoroughly discussed (see Helgesen, 1990; Wapenaar et al., 1990, 1992; Herrmann, 1992; Helgesen and Kolb, 1993; Amundsen, 1993). In the case of cylindrical symmetry this transformation constitutes a required preprocessing step for several seismic processing algorithms based on 2-D wave propagation. The work of Dampney (1971) has apparently been missed by the authors discussing themore » 3-D to 2-D geometric spreading transform. The purpose of the present work is to derive the necessary spatial filters for 2-D to 3-D transformation. The authors first consider the acoustic or elastic response of an explosive point source in a horizontally layered medium. Then the response of a point force in a horizontally layered elastic medium is considered. Finally, they give a numerical example where they apply the 2-D to 3-D transformation to an acoustic pressure field.« less