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Title: Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

Abstract

Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.

Authors:
 [1];  [2];  [1]; ORCiD logo [1]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biomedical and Diagnostic Sciences
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division. Mass Spectrometry and Laser Spectrometry
Publication Date:
Research Org.:
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; Tennessee Center of Excellence in Livestock Diseases and Human Health (United States)
OSTI Identifier:
1394588
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 8; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Staphylococcus aureus; sequence motif analysis; polymerase chain reaction; antibodies; flow cytometry; gene expression; protein expression; peptidoglycans

Citation Formats

Balachandran, Manasi, Giannone, Richard J., Bemis, David A., and Kania, Stephen A. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0183913.
Balachandran, Manasi, Giannone, Richard J., Bemis, David A., & Kania, Stephen A. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46. United States. https://doi.org/10.1371/journal.pone.0183913
Balachandran, Manasi, Giannone, Richard J., Bemis, David A., and Kania, Stephen A. 2017. "Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46". United States. https://doi.org/10.1371/journal.pone.0183913. https://www.osti.gov/servlets/purl/1394588.
@article{osti_1394588,
title = {Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46},
author = {Balachandran, Manasi and Giannone, Richard J. and Bemis, David A. and Kania, Stephen A.},
abstractNote = {Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.},
doi = {10.1371/journal.pone.0183913},
url = {https://www.osti.gov/biblio/1394588}, journal = {PLoS ONE},
issn = {1932-6203},
number = 8,
volume = 12,
place = {United States},
year = {Thu Aug 31 00:00:00 EDT 2017},
month = {Thu Aug 31 00:00:00 EDT 2017}
}

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Cited by: 4 works
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Works referenced in this record:

Bacterial cell wall-expressed protein A triggers supraclonal B-cell responses upon in vivo infection with Staphylococcus aureus
journal, December 2005


Confounding B-cell defences: lessons from a staphylococcal superantigen
journal, June 2006


Immune evasion by staphylococci
journal, December 2005


Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus : SORTASE CATALYZED
journal, March 2000


Use of protein A as an immunological reagent and its application using flow injection. A review
journal, January 1994


Virulence factor expression by Gram-positive cocci exposed to subinhibitory concentrations of linezolid
journal, September 2002


Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences
journal, March 2003


Indirect Visualization of Staphylococcus Aureus Protein a
journal, May 1970


Identifying protective antigens of Staphylococcus aureus , a pathogen that suppresses host immune responses
journal, July 2011


Protein secretion and surface display in Gram-positive bacteria
journal, April 2012


Function of the bacterial TATAAT -10 element as single-stranded DNA during RNA polymerase isomerization
journal, July 2001


Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments
journal, January 1970


Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
journal, May 2001


Formation of Extracellular Protein A by Staphylococcus aureus
journal, September 1976


Cloning and expression of the staphyiococcus aureus protein A gene in Escheirchia Coli
journal, January 1983


Antiinfective therapy with a small molecule inhibitor of Staphylococcus aureus sortase
journal, September 2014


Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus
journal, June 2001


Some Physicochemical Properties of Protein A from Staphylococcus aureus
journal, September 1972


Increased Infectivity of Staphylococcus aureus in an Experimental Model of Snake Venom–Induced Tissue Damage
journal, September 2007

  • Saravia‐Otten, Patricia; Gutiérrez, José María; Arvidson, Staffan
  • The Journal of Infectious Diseases, Vol. 196, Issue 5
  • https://doi.org/10.1086/520537

Latent LytM at 1.3Å Resolution
journal, January 2004


A Model B-Cell Superantigen and the Immunobiology of B Lymphocytes
journal, February 2002


Localization of Protein A in the Bacteria
journal, October 1972


Progress Toward a Staphylococcus aureus Vaccine
journal, December 2011


Surface-associated proteins of Staphylococcus aureus: Their possible roles in virulence
journal, May 1994


Characterization of immunoglobulin G binding to Staphylococcus aureus strain Wood 46
journal, December 1984

  • Amend, Alfred; Chhatwal, Gursharan S.; Schaeg, Werner
  • Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, Vol. 258, Issue 4
  • https://doi.org/10.1016/S0176-6724(84)80024-3

Staphylococcus aureus Infections
journal, August 1998


Use of staphylococcal protein A as an immunological reagent
journal, April 1978


Discovery of S taphylococcus aureus Sortase A Inhibitors Using Virtual Screening and the Relaxed Complex Scheme
journal, September 2013


Structure of the cell wall anchor of surface proteins in Staphylococcus aureus
journal, April 1995


Protein A Is Released into the Staphylococcus aureus Culture Supernatant with an Unprocessed Sorting Signal
journal, February 2015


Sortase as a Target of Anti-Infective Therapy
journal, March 2008


Release of protein A from the cell wall of Staphylococcus aureus
journal, January 2014


Nutritional Shifts sand their Effect on the Secretion of Extracellular Proteins by Staphylococcus aureus (Wood 46)
journal, April 1977


Recurrent infections and immune evasion strategies of Staphylococcus aureus
journal, February 2012


Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments
journal, January 1970


Use of staphylococcal protein A as an immunological reagent
journal, April 1978


Sorting of protein a to the staphylococcal cell wall
journal, July 1992


Latent LytM at 1.3Å Resolution
journal, January 2004


Recurrent infections and immune evasion strategies of Staphylococcus aureus
journal, February 2012


Bacterial cell wall-expressed protein A triggers supraclonal B-cell responses upon in vivo infection with Staphylococcus aureus
journal, December 2005


Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus
journal, January 2004


Confounding B-cell defences: lessons from a staphylococcal superantigen
journal, June 2006


Use of protein A as an immunological reagent and its application using flow injection. A review
journal, January 1994


Nutritional Shifts sand their Effect on the Secretion of Extracellular Proteins by Staphylococcus aureus (Wood 46)
journal, April 1977


Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus
journal, June 2001


Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
journal, May 2001


Antiinfective therapy with a small molecule inhibitor of Staphylococcus aureus sortase
journal, September 2014


Function of the bacterial TATAAT -10 element as single-stranded DNA during RNA polymerase isomerization
journal, July 2001


Indirect Visualization of Staphylococcus Aureus Protein a
journal, May 1970


Increased Infectivity of Staphylococcus aureus in an Experimental Model of Snake Venom–Induced Tissue Damage
journal, September 2007

  • Saravia‐Otten, Patricia; Gutiérrez, José María; Arvidson, Staffan
  • The Journal of Infectious Diseases, Vol. 196, Issue 5
  • https://doi.org/10.1086/520537

Progress Toward a Staphylococcus aureus Vaccine
journal, December 2011


Protein secretion and surface display in Gram-positive bacteria
journal, April 2012


Discovery of S taphylococcus aureus Sortase A Inhibitors Using Virtual Screening and the Relaxed Complex Scheme
journal, September 2013


Localization of Protein A in the Bacteria
journal, October 1972


Surface-associated proteins of Staphylococcus aureus: Their possible roles in virulence
journal, May 1994


Sortase as a Target of Anti-Infective Therapy
journal, March 2008


Structure of the cell wall anchor of surface proteins in Staphylococcus aureus
journal, April 1995


The Sbi Protein Is a Multifunctional Immune Evasion Factor of Staphylococcus aureus
journal, June 2011


Significance of Protein A Production by Staphylococci
journal, January 1970


Characterization of the Promoter Elements for the Staphylococcal Enterotoxin D Gene
journal, April 2000


Regulatory Elements of the Staphylococcus aureus Protein A (Spa) Promoter
journal, June 2004


Accessory Gene Regulator Control of Staphyloccoccal Enterotoxin D Gene Expression
journal, March 2004


Fibrinogen-binding proteins of Gram-positive bacteria
journal, January 2007