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Title: Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2

Abstract

Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells and whether they are transported to distal tissues. We used biologically active fluorophore and radiolabeled peptides to establish that flg22 moves to distal organs with the closest vascular connections. Remarkably, entry into the plant cell via endocytosis together with the FLS2 receptor is needed for delivery to vascular tissue and long-distance transport of flg22. This contrasts with known routes of long distance transport of other non-cell-permeant molecules in plants, which require membrane-localized transporters for entry to vascular tissue. Thus, a plasma membrane receptor acts as a transporter to enable access of its ligand to distal trafficking routes.

Authors:
 [1];  [2];  [3];  [2];  [1]
  1. Univ. of Chicago, IL (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1394455
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 68; Journal Issue: 7; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Jelenska, Joanna, Davern, Sandra M., Standaert, Robert F., Mirzadeh, Saed, and Greenberg, Jean T. Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2. United States: N. p., 2017. Web. doi:10.1093/jxb/erx060.
Jelenska, Joanna, Davern, Sandra M., Standaert, Robert F., Mirzadeh, Saed, & Greenberg, Jean T. Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2. United States. doi:10.1093/jxb/erx060.
Jelenska, Joanna, Davern, Sandra M., Standaert, Robert F., Mirzadeh, Saed, and Greenberg, Jean T. Wed . "Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2". United States. doi:10.1093/jxb/erx060. https://www.osti.gov/servlets/purl/1394455.
@article{osti_1394455,
title = {Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2},
author = {Jelenska, Joanna and Davern, Sandra M. and Standaert, Robert F. and Mirzadeh, Saed and Greenberg, Jean T.},
abstractNote = {Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells and whether they are transported to distal tissues. We used biologically active fluorophore and radiolabeled peptides to establish that flg22 moves to distal organs with the closest vascular connections. Remarkably, entry into the plant cell via endocytosis together with the FLS2 receptor is needed for delivery to vascular tissue and long-distance transport of flg22. This contrasts with known routes of long distance transport of other non-cell-permeant molecules in plants, which require membrane-localized transporters for entry to vascular tissue. Thus, a plasma membrane receptor acts as a transporter to enable access of its ligand to distal trafficking routes.},
doi = {10.1093/jxb/erx060},
journal = {Journal of Experimental Botany},
number = 7,
volume = 68,
place = {United States},
year = {Wed Mar 01 00:00:00 EST 2017},
month = {Wed Mar 01 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
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