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Title: Bacillus subtilis Lipid Extract, A Branched-Chain Fatty Acid Model Membrane

Abstract

Lipid extracts are an excellent choice of model biomembrane; however at present, there are no commercially available lipid extracts or computational models that mimic microbial membranes containing the branched-chain fatty acids found in many pathogenic and industrially relevant bacteria. Here, we advance the extract of Bacillus subtilis as a standard model for these diverse systems, providing a detailed experimental description and equilibrated atomistic bilayer model included as Supporting Information to this Letter and at (http://cmb.ornl.gov/members/cheng). The development and validation of this model represents an advance that enables more realistic simulations and experiments on bacterial membranes and reconstituted bacterial membrane proteins.

Authors:
ORCiD logo [1];  [2];  [3];  [2];  [4];  [4];  [5];  [2];  [6];  [7];  [8];  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Shull Wollan Center: A Joint Inst. for Neutron Sciences, Biology and Soft Matter Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Physics & Astronomy
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology and Soft Matter Division
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH
  5. Intel Corporation, Hillsboro, OR (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Shull Wollan Center: A Joint Inst. for Neutron Sciences, Biology and Soft Matter Division, Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biochemistry & Cellular and Molecular Biology
  7. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
  8. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biochemistry & Cellular and Molecular Biology
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Advanced Scientific Computing Research (ASCR) (SC-21)
OSTI Identifier:
1394446
DOE Contract Number:  
AC05-00OR22725; AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Journal of Physical Chemistry Letters
Additional Journal Information:
Journal Volume: 8; Journal Issue: 17; Journal ID: ISSN 1948-7185
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Nickels, Jonathan D., Chatterjee, Sneha, Mostofian, Barmak, Stanley, Christopher B., Ohl, Michael, Zolnierczuk, Piotr, Schulz, Roland, Myles, Dean A. A., Standaert, Robert F., Elkins, James G., Cheng, Xiaolin, and Katsaras, John. Bacillus subtilis Lipid Extract, A Branched-Chain Fatty Acid Model Membrane. United States: N. p., 2017. Web. doi:10.1021/acs.jpclett.7b01877.
Nickels, Jonathan D., Chatterjee, Sneha, Mostofian, Barmak, Stanley, Christopher B., Ohl, Michael, Zolnierczuk, Piotr, Schulz, Roland, Myles, Dean A. A., Standaert, Robert F., Elkins, James G., Cheng, Xiaolin, & Katsaras, John. Bacillus subtilis Lipid Extract, A Branched-Chain Fatty Acid Model Membrane. United States. doi:10.1021/acs.jpclett.7b01877.
Nickels, Jonathan D., Chatterjee, Sneha, Mostofian, Barmak, Stanley, Christopher B., Ohl, Michael, Zolnierczuk, Piotr, Schulz, Roland, Myles, Dean A. A., Standaert, Robert F., Elkins, James G., Cheng, Xiaolin, and Katsaras, John. Mon . "Bacillus subtilis Lipid Extract, A Branched-Chain Fatty Acid Model Membrane". United States. doi:10.1021/acs.jpclett.7b01877.
@article{osti_1394446,
title = {Bacillus subtilis Lipid Extract, A Branched-Chain Fatty Acid Model Membrane},
author = {Nickels, Jonathan D. and Chatterjee, Sneha and Mostofian, Barmak and Stanley, Christopher B. and Ohl, Michael and Zolnierczuk, Piotr and Schulz, Roland and Myles, Dean A. A. and Standaert, Robert F. and Elkins, James G. and Cheng, Xiaolin and Katsaras, John},
abstractNote = {Lipid extracts are an excellent choice of model biomembrane; however at present, there are no commercially available lipid extracts or computational models that mimic microbial membranes containing the branched-chain fatty acids found in many pathogenic and industrially relevant bacteria. Here, we advance the extract of Bacillus subtilis as a standard model for these diverse systems, providing a detailed experimental description and equilibrated atomistic bilayer model included as Supporting Information to this Letter and at (http://cmb.ornl.gov/members/cheng). The development and validation of this model represents an advance that enables more realistic simulations and experiments on bacterial membranes and reconstituted bacterial membrane proteins.},
doi = {10.1021/acs.jpclett.7b01877},
journal = {Journal of Physical Chemistry Letters},
issn = {1948-7185},
number = 17,
volume = 8,
place = {United States},
year = {2017},
month = {8}
}