Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction

Zhang, Ping; Wu, Wei-Min; Van Nostrand, Joy D.; Deng, Ye; He, Zhili; Gihring, Thomas; Zhang, Gengxin; Schadt, Chris W.; Watson, David; Jardine, Phil; Criddle, Craig S.; Brooks, Scott; Marsh, Terence L.; Tiedje, James M.; Arkin, Adam P.; Zhou, Jizhong

doi:10.1128/AEM.00043-15

Title: Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction

Journal Article · Fri Apr 10 00:00:00 EDT 2015 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.00043-15· OSTI ID:1394408

Zhang, Ping ^[1]; Wu, Wei-Min ^[2]; Van Nostrand, Joy D. ^[1]; Deng, Ye ^[1]; He, Zhili ^[1]; Gihring, Thomas ^[3]; Zhang, Gengxin ^[3]; Schadt, Chris W. ^[3]; Watson, David ^[3]; Jardine, Phil ^[3]; Criddle, Craig S. ^[2]; Brooks, Scott ^[3]; Marsh, Terence L. ^[4]; Tiedje, James M. ^[4]; Arkin, Adam P. ^[5]; Zhou, Jizhong ^[6]

Univ. of Oklahoma, Norman, OK (United States)
Stanford Univ., CA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Michigan State Univ., East Lansing, MI (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Oklahoma, Norman, OK (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Tsinghua Univ., Beijing (China)

A pilot-scale field experiment demonstrated that a one-time amendment of emulsified vegetable oil (EVO) reduced groundwater U(VI) concentrations for 1 year in a fast-flowing aquifer. However, little is known about how EVO amendment stimulates the functional gene composition, structure, and dynamics of groundwater microbial communities toward prolonged U(VI) reduction. In this study, we hypothesized that EVO amendment would shift the functional gene composition and structure of groundwater microbial communities and stimulate key functional genes/groups involved in EVO biodegradation and reduction of electron acceptors in the aquifer. To test these hypotheses, groundwater microbial communities after EVO amendment were analyzed using a comprehensive functional gene microarray. Our results showed that EVO amendment stimulated sequential shifts in the functional composition and structure of groundwater microbial communities. Particularly, the relative abundance of key functional genes/groups involved in EVO biodegradation and the reduction of NO₃^-, Mn(IV), Fe(III), U(VI), and SO₄^2-significantly increased, especially during the active U(VI) reduction period. The relative abundance for some of these key functional genes/groups remained elevated over 9 months. Montel tests suggested that the dynamics in the abundance, composition, and structure of these key functional genes/groups were significantly correlated with groundwater concentrations of acetate, NO₃^-, Mn(II), Fe(II), U(VI), and SO₄^2-. Our results suggest that EVO amendment stimulated dynamic succession of key functional microbial communities. This study improves our understanding of the composition, structure, and function changes needed for groundwater microbial communities to sustain a long-term U(VI) reduction.

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Research Organization:: Univ. of Oklahoma, Norman, OK (United States); Michigan State Univ., East Lansing, MI (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231; FG02-07ER64398

OSTI ID:: 1394408

Alternate ID(s):: OSTI ID: 1511425

Journal Information:: Applied and Environmental Microbiology, Vol. 81, Issue 12; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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