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Title: The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum

Abstract

Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity. The four genes alone, however, were insufficient to achieve in C. thermocellum the ethanol yields and titers observed in engineered T. saccharolyticum strains, even when combined with gene deletions targeting hydrogen production. Here, this suggests that other parts of T. saccharolyticum metabolism may also be necessary to reproduce the high ethanol yield and titer phenotype in C. thermocellum.

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [2];  [1]
  1. Dartmouth College, Hanover, NH (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Dartmouth College, Hanover, NH (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1394382
Alternate Identifier(s):
OSTI ID: 1367525
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Metabolic Engineering
Additional Journal Information:
Journal Volume: 42; Journal Issue: C; Journal ID: ISSN 1096-7176
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Hon, Shuen, Olson, Daniel G., Holwerda, Evert K., Lanahan, Anthony A., Murphy, Sean J. L., Maloney, Marybeth I., Zheng, Tianyong, Papanek, Beth, Guss, Adam M., and Lynd, Lee R. The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum. United States: N. p., 2017. Web. doi:10.1016/j.ymben.2017.06.011.
Hon, Shuen, Olson, Daniel G., Holwerda, Evert K., Lanahan, Anthony A., Murphy, Sean J. L., Maloney, Marybeth I., Zheng, Tianyong, Papanek, Beth, Guss, Adam M., & Lynd, Lee R. The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum. United States. doi:10.1016/j.ymben.2017.06.011.
Hon, Shuen, Olson, Daniel G., Holwerda, Evert K., Lanahan, Anthony A., Murphy, Sean J. L., Maloney, Marybeth I., Zheng, Tianyong, Papanek, Beth, Guss, Adam M., and Lynd, Lee R. Tue . "The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum". United States. doi:10.1016/j.ymben.2017.06.011. https://www.osti.gov/servlets/purl/1394382.
@article{osti_1394382,
title = {The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum},
author = {Hon, Shuen and Olson, Daniel G. and Holwerda, Evert K. and Lanahan, Anthony A. and Murphy, Sean J. L. and Maloney, Marybeth I. and Zheng, Tianyong and Papanek, Beth and Guss, Adam M. and Lynd, Lee R.},
abstractNote = {Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity. The four genes alone, however, were insufficient to achieve in C. thermocellum the ethanol yields and titers observed in engineered T. saccharolyticum strains, even when combined with gene deletions targeting hydrogen production. Here, this suggests that other parts of T. saccharolyticum metabolism may also be necessary to reproduce the high ethanol yield and titer phenotype in C. thermocellum.},
doi = {10.1016/j.ymben.2017.06.011},
journal = {Metabolic Engineering},
number = C,
volume = 42,
place = {United States},
year = {Tue Jun 27 00:00:00 EDT 2017},
month = {Tue Jun 27 00:00:00 EDT 2017}
}

Journal Article:
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Cited by: 6 works
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