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Title: New technologies accelerate the exploration of non-coding RNAs in horticultural plants

Abstract

Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small portion of the genome space in plants, the universe of plant ncRNAs is rapidly expanding. Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs. Here we summarize the classification and known biological functions of plant ncRNAs, review the application of next-generation sequencing (NGS) technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies, especially the new genome-editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems, to functional characterization of plant ncRNAs.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1393820
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Horticulture Research; Journal Volume: 4; Journal Issue: 17031
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Liu, Degao, Mewalal, Ritesh, Hu, Rongbin, Tuskan, Gerald A., and Yang, Xiaohan. New technologies accelerate the exploration of non-coding RNAs in horticultural plants. United States: N. p., 2017. Web. doi:10.1038/hortres.2017.31.
Liu, Degao, Mewalal, Ritesh, Hu, Rongbin, Tuskan, Gerald A., & Yang, Xiaohan. New technologies accelerate the exploration of non-coding RNAs in horticultural plants. United States. doi:10.1038/hortres.2017.31.
Liu, Degao, Mewalal, Ritesh, Hu, Rongbin, Tuskan, Gerald A., and Yang, Xiaohan. Wed . "New technologies accelerate the exploration of non-coding RNAs in horticultural plants". United States. doi:10.1038/hortres.2017.31.
@article{osti_1393820,
title = {New technologies accelerate the exploration of non-coding RNAs in horticultural plants},
author = {Liu, Degao and Mewalal, Ritesh and Hu, Rongbin and Tuskan, Gerald A. and Yang, Xiaohan},
abstractNote = {Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small portion of the genome space in plants, the universe of plant ncRNAs is rapidly expanding. Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs. Here we summarize the classification and known biological functions of plant ncRNAs, review the application of next-generation sequencing (NGS) technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies, especially the new genome-editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems, to functional characterization of plant ncRNAs.},
doi = {10.1038/hortres.2017.31},
journal = {Horticulture Research},
number = 17031,
volume = 4,
place = {United States},
year = {Wed Jul 05 00:00:00 EDT 2017},
month = {Wed Jul 05 00:00:00 EDT 2017}
}