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Title: Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms

Abstract

Modern live-cell imaging approaches permit real-time visualization of biological processes, yet limitations exist for unicellular organism isolation, culturing and long-term imaging that preclude fully understanding how cells sense and respond to environmental perturbations and the link between single-cell variability and whole-population dynamics. Here we present a microfluidic platform that provides fine control over the local environment with the capacity to replace media components at any experimental time point, and provides both perfused and compartmentalized cultivation conditions depending on the valve configuration. The functionality and flexibility of the platform were validated using both bacteria and yeast having different sizes, motility and growth media. The demonstrated ability to track the growth and dynamics of both motile and non-motile prokaryotic and eukaryotic organisms emphasizes the versatility of the devices, which with further scale-up should enable studies in bioenergy and environmental research.

Authors:
; ; ; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1393736
Report Number(s):
PNNL-SA-129125
Journal ID: ISSN 1932-1058; 49612; KP1606000
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biomicrofluidics; Journal Volume: 11; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Geng, Tao, Smallwood, Chuck R., Bredeweg, Erin L., Pomraning, Kyle R., Plymale, Andrew E., Baker, Scott E., Evans, James E., and Kelly, Ryan T. Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms. United States: N. p., 2017. Web. doi:10.1063/1.4986533.
Geng, Tao, Smallwood, Chuck R., Bredeweg, Erin L., Pomraning, Kyle R., Plymale, Andrew E., Baker, Scott E., Evans, James E., & Kelly, Ryan T. Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms. United States. doi:10.1063/1.4986533.
Geng, Tao, Smallwood, Chuck R., Bredeweg, Erin L., Pomraning, Kyle R., Plymale, Andrew E., Baker, Scott E., Evans, James E., and Kelly, Ryan T. Fri . "Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms". United States. doi:10.1063/1.4986533.
@article{osti_1393736,
title = {Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms},
author = {Geng, Tao and Smallwood, Chuck R. and Bredeweg, Erin L. and Pomraning, Kyle R. and Plymale, Andrew E. and Baker, Scott E. and Evans, James E. and Kelly, Ryan T.},
abstractNote = {Modern live-cell imaging approaches permit real-time visualization of biological processes, yet limitations exist for unicellular organism isolation, culturing and long-term imaging that preclude fully understanding how cells sense and respond to environmental perturbations and the link between single-cell variability and whole-population dynamics. Here we present a microfluidic platform that provides fine control over the local environment with the capacity to replace media components at any experimental time point, and provides both perfused and compartmentalized cultivation conditions depending on the valve configuration. The functionality and flexibility of the platform were validated using both bacteria and yeast having different sizes, motility and growth media. The demonstrated ability to track the growth and dynamics of both motile and non-motile prokaryotic and eukaryotic organisms emphasizes the versatility of the devices, which with further scale-up should enable studies in bioenergy and environmental research.},
doi = {10.1063/1.4986533},
journal = {Biomicrofluidics},
number = 5,
volume = 11,
place = {United States},
year = {Fri Sep 01 00:00:00 EDT 2017},
month = {Fri Sep 01 00:00:00 EDT 2017}
}